Human Herpesvirus 6 Detection in Alzheimer's Disease Cases and Controls across Multiple Cohorts

Mary Alice Allnutt; Kory Johnson; David A. Bennett; Sarah M. Connor; Juan C. Troncoso; Olga Pletnikova; Marilyn S. Albert; Susan M. Resnick; Sonja W. Scholz; Philip L. De Jager; Steven Jacobson

doi:10.1016/j.neuron.2019.12.031

Human Herpesvirus 6 Detection in Alzheimer's Disease Cases and Controls across Multiple Cohorts

Mary Alice Allnutt, Kory Johnson, David A. Bennett, Sarah M. Connor, Juan C. Troncoso, Olga Pletnikova, Marilyn S. Albert, Susan M. Resnick, Sonja W. Scholz, Philip L. De Jager, Steven Jacobson

School of Medicine

Research output: Contribution to journal › Article › peer-review

24 Scopus citations

Abstract

The interplay between viral infection and Alzheimer's disease (AD) has long been an area of interest, but proving causality has been elusive. Several recent studies have renewed the debate concerning the role of herpesviruses, and human herpesvirus 6 (HHV-6) in particular, in AD. We screened for HHV-6 detection across three independent AD brain repositories using (1) RNA sequencing (RNA-seq) datasets and (2) DNA samples extracted from AD and non-AD control brains. The RNA-seq data were screened for pathogens against taxon references from over 25,000 microbes, including 118 human viruses, whereas DNA samples were probed for PCR reactivity to HHV-6A and HHV-6B. HHV-6 demonstrated little specificity to AD brains over controls by either method, whereas other viruses, such as Epstein-Barr virus (EBV) and cytomegalovirus (CMV), were detected at comparable levels. These direct methods of viral detection do not suggest an association between HHV-6 and AD.

Original language	English (US)
Pages (from-to)	1027-1035.e2
Journal	Neuron
Volume	105
Issue number	6
DOIs	https://doi.org/10.1016/j.neuron.2019.12.031
State	Published - Mar 18 2020

Keywords

Alzheimer's disease
herpesvirus
human herpesvirus 6

ASJC Scopus subject areas

General Neuroscience

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10.1016/j.neuron.2019.12.031

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title = "Human Herpesvirus 6 Detection in Alzheimer's Disease Cases and Controls across Multiple Cohorts",

abstract = "The interplay between viral infection and Alzheimer's disease (AD) has long been an area of interest, but proving causality has been elusive. Several recent studies have renewed the debate concerning the role of herpesviruses, and human herpesvirus 6 (HHV-6) in particular, in AD. We screened for HHV-6 detection across three independent AD brain repositories using (1) RNA sequencing (RNA-seq) datasets and (2) DNA samples extracted from AD and non-AD control brains. The RNA-seq data were screened for pathogens against taxon references from over 25,000 microbes, including 118 human viruses, whereas DNA samples were probed for PCR reactivity to HHV-6A and HHV-6B. HHV-6 demonstrated little specificity to AD brains over controls by either method, whereas other viruses, such as Epstein-Barr virus (EBV) and cytomegalovirus (CMV), were detected at comparable levels. These direct methods of viral detection do not suggest an association between HHV-6 and AD.",

keywords = "Alzheimer's disease, herpesvirus, human herpesvirus 6",

author = "Allnutt, {Mary Alice} and Kory Johnson and Bennett, {David A.} and Connor, {Sarah M.} and Troncoso, {Juan C.} and Olga Pletnikova and Albert, {Marilyn S.} and Resnick, {Susan M.} and Scholz, {Sonja W.} and {De Jager}, {Philip L.} and Steven Jacobson",

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doi = "10.1016/j.neuron.2019.12.031",

language = "English (US)",

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AU - Johnson, Kory

AU - Bennett, David A.

AU - Connor, Sarah M.

AU - Troncoso, Juan C.

AU - Pletnikova, Olga

AU - Albert, Marilyn S.

AU - Resnick, Susan M.

AU - Scholz, Sonja W.

AU - De Jager, Philip L.

AU - Jacobson, Steven

PY - 2020/3/18

Y1 - 2020/3/18

N2 - The interplay between viral infection and Alzheimer's disease (AD) has long been an area of interest, but proving causality has been elusive. Several recent studies have renewed the debate concerning the role of herpesviruses, and human herpesvirus 6 (HHV-6) in particular, in AD. We screened for HHV-6 detection across three independent AD brain repositories using (1) RNA sequencing (RNA-seq) datasets and (2) DNA samples extracted from AD and non-AD control brains. The RNA-seq data were screened for pathogens against taxon references from over 25,000 microbes, including 118 human viruses, whereas DNA samples were probed for PCR reactivity to HHV-6A and HHV-6B. HHV-6 demonstrated little specificity to AD brains over controls by either method, whereas other viruses, such as Epstein-Barr virus (EBV) and cytomegalovirus (CMV), were detected at comparable levels. These direct methods of viral detection do not suggest an association between HHV-6 and AD.

AB - The interplay between viral infection and Alzheimer's disease (AD) has long been an area of interest, but proving causality has been elusive. Several recent studies have renewed the debate concerning the role of herpesviruses, and human herpesvirus 6 (HHV-6) in particular, in AD. We screened for HHV-6 detection across three independent AD brain repositories using (1) RNA sequencing (RNA-seq) datasets and (2) DNA samples extracted from AD and non-AD control brains. The RNA-seq data were screened for pathogens against taxon references from over 25,000 microbes, including 118 human viruses, whereas DNA samples were probed for PCR reactivity to HHV-6A and HHV-6B. HHV-6 demonstrated little specificity to AD brains over controls by either method, whereas other viruses, such as Epstein-Barr virus (EBV) and cytomegalovirus (CMV), were detected at comparable levels. These direct methods of viral detection do not suggest an association between HHV-6 and AD.

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KW - human herpesvirus 6

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Human Herpesvirus 6 Detection in Alzheimer's Disease Cases and Controls across Multiple Cohorts

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