Human fibroblast reprogramming to pluripotent stem cells regulated by the miR19a/b-PTEN axis

Xiaoping He, Yang Cao, Lihua Wang, Yingli Han, Xiuying Zhong, Guixiang Zhou, Yongping Cai, Huafeng Zhang, Ping Gao

Research output: Contribution to journalArticle

Abstract

Induction of pluripotent stem cells (iPSC) by defined transcription factors is the recognized canonical means for somatic reprogramming, however, it remains incompletely understood how individual transcription factors affect cell fate decisions during the reprogramming process. Here, we report induction of fibroblast reprogramming by various transcriptional factors is mediated by a miR19a/b-PTEN axis. cMyc, one of the four Yamanaka factors known to stimulate both somatic cell reprogramming and tumorigenesis, induced the expression of multiple mircoRNAs, miR-17∼92 cluster in particular, in the early stage of reprogramming of human fibroblasts. Importantly, miR-17,92 cluster could greatly enhance human fibroblast reprogramming induced by either the four Yamanaka factors (Oct4, Sox2, Klf4, and cMyc, or 4F) or the first three transcriptional factors (Oct4, Sox2, and Klf4, or 3F). Among members of this microRNA cluster, miR-19a/b exhibited the most potent effect on stimulating fibroblst reprogramming to iPSCs. Additional studies revealed that miR-19a/b enhanced iPSC induction efficiency by targeted inhibition of phosphatase and tensin homolog (PTEN), a renowned tumor suppressor whose loss-of-function mutations were found in multiple human malignancies. Our results thus demonstrate an important role of miR-19a/b-PTEN axis in the reprogramming of human fibroblasts, illustrating that the somatic reprogramming process and its underlying regulation pathways are intertwined with oncogenic signaling in human malignancies.

Original languageEnglish (US)
Article numbere95213
JournalPLoS One
Volume9
Issue number4
DOIs
StatePublished - Apr 1 2014
Externally publishedYes

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Pluripotent Stem Cells
Fibroblasts
Stem cells
Phosphoric Monoester Hydrolases
fibroblasts
stem cells
transcription factors
Transcription Factors
Neoplasms
MicroRNAs
Tumors
microRNA
somatic cells
carcinogenesis
Carcinogenesis
Tensins
Mutation
neoplasms
cells

ASJC Scopus subject areas

  • Agricultural and Biological Sciences(all)
  • Biochemistry, Genetics and Molecular Biology(all)
  • Medicine(all)

Cite this

He, X., Cao, Y., Wang, L., Han, Y., Zhong, X., Zhou, G., ... Gao, P. (2014). Human fibroblast reprogramming to pluripotent stem cells regulated by the miR19a/b-PTEN axis. PLoS One, 9(4), [e95213]. https://doi.org/10.1371/journal.pone.0095213

Human fibroblast reprogramming to pluripotent stem cells regulated by the miR19a/b-PTEN axis. / He, Xiaoping; Cao, Yang; Wang, Lihua; Han, Yingli; Zhong, Xiuying; Zhou, Guixiang; Cai, Yongping; Zhang, Huafeng; Gao, Ping.

In: PLoS One, Vol. 9, No. 4, e95213, 01.04.2014.

Research output: Contribution to journalArticle

He, X, Cao, Y, Wang, L, Han, Y, Zhong, X, Zhou, G, Cai, Y, Zhang, H & Gao, P 2014, 'Human fibroblast reprogramming to pluripotent stem cells regulated by the miR19a/b-PTEN axis', PLoS One, vol. 9, no. 4, e95213. https://doi.org/10.1371/journal.pone.0095213
He, Xiaoping ; Cao, Yang ; Wang, Lihua ; Han, Yingli ; Zhong, Xiuying ; Zhou, Guixiang ; Cai, Yongping ; Zhang, Huafeng ; Gao, Ping. / Human fibroblast reprogramming to pluripotent stem cells regulated by the miR19a/b-PTEN axis. In: PLoS One. 2014 ; Vol. 9, No. 4.
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