Human enteroids/colonoids and intestinal organoids functionally recapitulate normal intestinal physiology and pathophysiology

Nicholas C. Zachos, Olga Kovbasnjuk, Jennifer Foulke-Abel, Julie In, Sarah E. Blutt, Hugo R. De Jonge, Mary K. Estes, Mark Donowitz

Research output: Contribution to journalArticle

Abstract

Identification of Lgr5 as the intestinal stem cell markeras well as the growth factors necessary to replicate adult intestinal stem cell division has led to the establishment of the methods to generate "indefinite" ex vivo primary intestinal epithelial cultures, termed "mini-intestines." Primary cultures developed from isolated intestinal crypts or stem cells (termed enteroids/colonoids) and from inducible pluripotent stem cells (termed intestinal organoids) are being applied to study human intestinal physiology and pathophysiology with great expectations for translational applications, including regenerative medicine. Here we discuss the physiologic properties of these cultures, their current use in understanding diarrhea-causing host-pathogen interactions, and potential future applications.

Original languageEnglish (US)
Pages (from-to)3759-3766
Number of pages8
JournalJournal of Biological Chemistry
Volume291
Issue number8
DOIs
StatePublished - Feb 19 2016

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ASJC Scopus subject areas

  • Biochemistry
  • Cell Biology
  • Molecular Biology

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