Human enteroids: Preclinical models of non-inflammatory diarrhea

Olga Kovbasnjuk; Nicholas C. Zachos; Julie In; Jennifer Foulke-Abel; Khalil Ettayebi; Joseph M. Hyser; James R. Broughman; Xi Lei Zeng; Sabine Middendorp; Hugo R. De Jonge; Mary K. Estes; Mark Donowitz

doi:10.1186/scrt364

Human enteroids: Preclinical models of non-inflammatory diarrhea

Olga Kovbasnjuk, Nicholas C. Zachos, Julie In, Jennifer Foulke-Abel, Khalil Ettayebi, Joseph M. Hyser, James R. Broughman, Xi Lei Zeng, Sabine Middendorp, Hugo R. De Jonge, Mary K. Estes, Mark Donowitz

School of Medicine

Research output: Contribution to journal › Review article › peer-review

35 Scopus citations

Abstract

Researchers need an available and easy-to-use model of the human intestine to better understand human intestinal physiology and pathophysiology of diseases, and to offer an enhanced platform for developing drug therapy. Our work employs human enteroids derived from each of the major intestinal sections to advance understanding of several diarrheal diseases, including those caused by cholera, rotavirus and enterohemorrhagic Escherichia coli. An enteroid bank is being established to facilitate comparison of segmental, developmental, and regulatory differences in transport proteins that can influence therapy efficacy. Basic characterization of major ion transport protein expression, localization and function in the human enteroid model sets the stage to study the effects of enteric infection at the transport level, as well as to monitor potential responses to pharmacological intervention.

Original language	English (US)
Article number	S3
Journal	Stem Cell Research and Therapy
Volume	4
Issue number	SUPPL.1
DOIs	https://doi.org/10.1186/scrt364
State	Published - Dec 20 2013

ASJC Scopus subject areas

Medicine (miscellaneous)
Molecular Medicine
Biochemistry, Genetics and Molecular Biology (miscellaneous)
Cell Biology

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title = "Human enteroids: Preclinical models of non-inflammatory diarrhea",

abstract = "Researchers need an available and easy-to-use model of the human intestine to better understand human intestinal physiology and pathophysiology of diseases, and to offer an enhanced platform for developing drug therapy. Our work employs human enteroids derived from each of the major intestinal sections to advance understanding of several diarrheal diseases, including those caused by cholera, rotavirus and enterohemorrhagic Escherichia coli. An enteroid bank is being established to facilitate comparison of segmental, developmental, and regulatory differences in transport proteins that can influence therapy efficacy. Basic characterization of major ion transport protein expression, localization and function in the human enteroid model sets the stage to study the effects of enteric infection at the transport level, as well as to monitor potential responses to pharmacological intervention.",

author = "Olga Kovbasnjuk and Zachos, {Nicholas C.} and Julie In and Jennifer Foulke-Abel and Khalil Ettayebi and Hyser, {Joseph M.} and Broughman, {James R.} and Zeng, {Xi Lei} and Sabine Middendorp and {De Jonge}, {Hugo R.} and Estes, {Mary K.} and Mark Donowitz",

note = "Funding Information: This work was supported in part by National Institutes of Health NCATS and NIDDK Grants U18-TR000552 (NCATS), R01-DK26523, R01-DK61765, P01-DK072084, K08-DK088950, R01-AI080656 and P30-DK56336 (Hopkins), and P30-DK089502 (Baylor). The authors acknowledge use of the Kudsi Imaging Core Facility of the Conte Hopkins Digestive Disease Basic & Translational Research Core Center. This study was approved by the Johns Hopkins University School of Medicine and Baylor College of Medicine Institutional Review Boards. The publication costs for this article were funded through NIH NCATS grant U18-TR000552.",

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AU - Kovbasnjuk, Olga

AU - Zachos, Nicholas C.

AU - In, Julie

AU - Foulke-Abel, Jennifer

AU - Ettayebi, Khalil

AU - Hyser, Joseph M.

AU - Broughman, James R.

AU - Zeng, Xi Lei

AU - Middendorp, Sabine

AU - De Jonge, Hugo R.

AU - Estes, Mary K.

AU - Donowitz, Mark

N1 - Funding Information: This work was supported in part by National Institutes of Health NCATS and NIDDK Grants U18-TR000552 (NCATS), R01-DK26523, R01-DK61765, P01-DK072084, K08-DK088950, R01-AI080656 and P30-DK56336 (Hopkins), and P30-DK089502 (Baylor). The authors acknowledge use of the Kudsi Imaging Core Facility of the Conte Hopkins Digestive Disease Basic & Translational Research Core Center. This study was approved by the Johns Hopkins University School of Medicine and Baylor College of Medicine Institutional Review Boards. The publication costs for this article were funded through NIH NCATS grant U18-TR000552.

PY - 2013/12/20

Y1 - 2013/12/20

N2 - Researchers need an available and easy-to-use model of the human intestine to better understand human intestinal physiology and pathophysiology of diseases, and to offer an enhanced platform for developing drug therapy. Our work employs human enteroids derived from each of the major intestinal sections to advance understanding of several diarrheal diseases, including those caused by cholera, rotavirus and enterohemorrhagic Escherichia coli. An enteroid bank is being established to facilitate comparison of segmental, developmental, and regulatory differences in transport proteins that can influence therapy efficacy. Basic characterization of major ion transport protein expression, localization and function in the human enteroid model sets the stage to study the effects of enteric infection at the transport level, as well as to monitor potential responses to pharmacological intervention.

AB - Researchers need an available and easy-to-use model of the human intestine to better understand human intestinal physiology and pathophysiology of diseases, and to offer an enhanced platform for developing drug therapy. Our work employs human enteroids derived from each of the major intestinal sections to advance understanding of several diarrheal diseases, including those caused by cholera, rotavirus and enterohemorrhagic Escherichia coli. An enteroid bank is being established to facilitate comparison of segmental, developmental, and regulatory differences in transport proteins that can influence therapy efficacy. Basic characterization of major ion transport protein expression, localization and function in the human enteroid model sets the stage to study the effects of enteric infection at the transport level, as well as to monitor potential responses to pharmacological intervention.

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Human enteroids: Preclinical models of non-inflammatory diarrhea

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