Human embryonic stem cell-derived hematoendothelial progenitors engraft chicken embryos

Tea Soon Park; Elias Zambidis; Jennifer L. Lucitti; Alison Logar; Bradley B. Keller; Bruno Péault

doi:10.1016/j.exphem.2008.08.007

Human embryonic stem cell-derived hematoendothelial progenitors engraft chicken embryos

Tea Soon Park, Elias Zambidis, Jennifer L. Lucitti, Alison Logar, Bradley B. Keller, Bruno Péault

School of Medicine

Original language	English (US)
Pages (from-to)	31-41
Number of pages	11
Journal	Experimental Hematology
Volume	37
Issue number	1
DOIs	https://doi.org/10.1016/j.exphem.2008.08.007
Publication status	Published - Jan 2009

ASJC Scopus subject areas

Cancer Research
Cell Biology
Genetics
Molecular Biology
Hematology

Cite this

Park, T. S., Zambidis, E., Lucitti, J. L., Logar, A., Keller, B. B., & Péault, B. (2009). Human embryonic stem cell-derived hematoendothelial progenitors engraft chicken embryos. Experimental Hematology, 37(1), 31-41. https://doi.org/10.1016/j.exphem.2008.08.007

@article{fef895ecbd4740afb74b997ee832cb73,

title = "Human embryonic stem cell-derived hematoendothelial progenitors engraft chicken embryos",

abstract = "Objective: To investigate whether human embryonic stem cells (hESC) committed in culture into hematopoietic/endothelial cell progenitors can be further developed into mature blood and vascular cells following transplantation into chicken embryos. Materials and Methods: The yolk sac of 42- to 44-hour chicken embryos received yolk sac injections of unfractionated human embryoid body (hEB) cells, CD34-positive hEB cells, or CD34+CD45+ granulocyte colony-stimulating factor-mobilized human peripheral blood hematopoietic stem-progenitor cells. Human cells in the host were detected by flow cytometry and immunohistochemistry. Results: All injected cell populations engrafted chicken hematopoietic organs, as assessed by detection of CD45+ cells in the spleen, bursa of Fabricius, and thymus. CD34+ day -10 hEB cells showed the highest efficiency for producing human CD45+ cells in the hosts and yielded human glycophorin A+ erythroid, CD13+ myeloid, and CD19+ lymphoid cells in the spleen and bursa of Fabricius. Spleen cells from chimeric embryos also contained human colony-forming units-granulocyte macrophage, as assessed in methylcellulose colony-forming assays. Human endothelial cells expressing vascular endothelial-cadherin, von Willebrand factor, CD31, and the receptor for the Ulex europaeus lectin were also observed in the yolk sac vasculature following injection of either unfractionated or CD34+ day -10 hEB cells. Conclusion: Primitive angiohematopoietic stem cells (total and CD34+ day -10 hEB cells) as well as adult hematopoietic stem cells could home to intraembryonic blood-forming organs following injection into the yolk sac. These observations demonstrate the utility of the avian embryo as a convenient and reliable host to model the angiohematopoietic development of human embryonic, or other early stem cells.",

author = "Park, {Tea Soon} and Elias Zambidis and Lucitti, {Jennifer L.} and Alison Logar and Keller, {Bradley B.} and Bruno P{\'e}ault",

year = "2009",

month = "1",

doi = "10.1016/j.exphem.2008.08.007",

language = "English (US)",

volume = "37",

pages = "31--41",

journal = "Experimental Hematology",

issn = "0301-472X",

publisher = "Elsevier Inc.",

number = "1",

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TY - JOUR

T1 - Human embryonic stem cell-derived hematoendothelial progenitors engraft chicken embryos

AU - Park, Tea Soon

AU - Zambidis, Elias

AU - Lucitti, Jennifer L.

AU - Logar, Alison

AU - Keller, Bradley B.

AU - Péault, Bruno

PY - 2009/1

Y1 - 2009/1

N2 - Objective: To investigate whether human embryonic stem cells (hESC) committed in culture into hematopoietic/endothelial cell progenitors can be further developed into mature blood and vascular cells following transplantation into chicken embryos. Materials and Methods: The yolk sac of 42- to 44-hour chicken embryos received yolk sac injections of unfractionated human embryoid body (hEB) cells, CD34-positive hEB cells, or CD34+CD45+ granulocyte colony-stimulating factor-mobilized human peripheral blood hematopoietic stem-progenitor cells. Human cells in the host were detected by flow cytometry and immunohistochemistry. Results: All injected cell populations engrafted chicken hematopoietic organs, as assessed by detection of CD45+ cells in the spleen, bursa of Fabricius, and thymus. CD34+ day -10 hEB cells showed the highest efficiency for producing human CD45+ cells in the hosts and yielded human glycophorin A+ erythroid, CD13+ myeloid, and CD19+ lymphoid cells in the spleen and bursa of Fabricius. Spleen cells from chimeric embryos also contained human colony-forming units-granulocyte macrophage, as assessed in methylcellulose colony-forming assays. Human endothelial cells expressing vascular endothelial-cadherin, von Willebrand factor, CD31, and the receptor for the Ulex europaeus lectin were also observed in the yolk sac vasculature following injection of either unfractionated or CD34+ day -10 hEB cells. Conclusion: Primitive angiohematopoietic stem cells (total and CD34+ day -10 hEB cells) as well as adult hematopoietic stem cells could home to intraembryonic blood-forming organs following injection into the yolk sac. These observations demonstrate the utility of the avian embryo as a convenient and reliable host to model the angiohematopoietic development of human embryonic, or other early stem cells.

AB - Objective: To investigate whether human embryonic stem cells (hESC) committed in culture into hematopoietic/endothelial cell progenitors can be further developed into mature blood and vascular cells following transplantation into chicken embryos. Materials and Methods: The yolk sac of 42- to 44-hour chicken embryos received yolk sac injections of unfractionated human embryoid body (hEB) cells, CD34-positive hEB cells, or CD34+CD45+ granulocyte colony-stimulating factor-mobilized human peripheral blood hematopoietic stem-progenitor cells. Human cells in the host were detected by flow cytometry and immunohistochemistry. Results: All injected cell populations engrafted chicken hematopoietic organs, as assessed by detection of CD45+ cells in the spleen, bursa of Fabricius, and thymus. CD34+ day -10 hEB cells showed the highest efficiency for producing human CD45+ cells in the hosts and yielded human glycophorin A+ erythroid, CD13+ myeloid, and CD19+ lymphoid cells in the spleen and bursa of Fabricius. Spleen cells from chimeric embryos also contained human colony-forming units-granulocyte macrophage, as assessed in methylcellulose colony-forming assays. Human endothelial cells expressing vascular endothelial-cadherin, von Willebrand factor, CD31, and the receptor for the Ulex europaeus lectin were also observed in the yolk sac vasculature following injection of either unfractionated or CD34+ day -10 hEB cells. Conclusion: Primitive angiohematopoietic stem cells (total and CD34+ day -10 hEB cells) as well as adult hematopoietic stem cells could home to intraembryonic blood-forming organs following injection into the yolk sac. These observations demonstrate the utility of the avian embryo as a convenient and reliable host to model the angiohematopoietic development of human embryonic, or other early stem cells.

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10.1016/j.exphem.2008.08.007