Human embryoid body-derived stem cells in bladder regeneration using rodent model

Dominic Frimberger, Nelly Morales, Michael Shamblott, John D. Gearhart, John Phillip Gearhart, Yegappan Lakshmanan

Research output: Contribution to journalArticle

Abstract

Objectives. To evaluate the capability of a human embryonic germ (hEG) cell-derived cell line (SDEC), previously characterized in our laboratory, seeded on porcine small intestinal submucosa (SIS) to regenerate the injured rat bladder. Methods. Fluorescent-labeled SDEC cells seeded on SIS for 8 days in vitro were used as bladder grafts in rats. A total of 30 congenitally athymic rats (six groups of 5 rats each), underwent partial cystectomy and replacement with plain SIS (groups 1 to 3) or cell-seeded SIS (groups 4 to 6). The rats were sacrificed after 7 (groups 1 and 4), 14 (groups 2 and 5), and 28 (groups 3 and 6) days. The bladders were analyzed by histopathologic examination and fluorescence microscopy. Results. No graft rejection or diminution in bladder capacity occurred. Plain SIS implants had multiple calcareous deposits, not seen with the cell-seeded implants. Macroscopically, at 7 days, the grafts were healed with a cellular lining on the luminal aspect in groups 4 to 6. Microscopically, the rat bladder was completely regenerated 28 days after stem cell-seeded SIS implantation. Labeled stem cells were identified throughout the graft and contributed significantly to bladder regeneration. Conclusions. The results of this study have demonstrated the successful replacement of a bladder defect in a rat model using hEG cell-derived cells seeded on SIS grafts. Longer term analysis of these bladder grafts will allow evaluation of function, cell migration, and differentiation processes of human stem cells.

Original languageEnglish (US)
Pages (from-to)827-832
Number of pages6
JournalUrology
Volume65
Issue number4
DOIs
StatePublished - Apr 2005

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Embryoid Bodies
Human Body
Regeneration
Rodentia
Urinary Bladder
Stem Cells
Transplants
Nude Rats
Cystectomy
Graft Rejection
Fluorescence Microscopy
Cell Movement
Cell Differentiation
Swine
Cell Line

ASJC Scopus subject areas

  • Urology

Cite this

Frimberger, D., Morales, N., Shamblott, M., Gearhart, J. D., Gearhart, J. P., & Lakshmanan, Y. (2005). Human embryoid body-derived stem cells in bladder regeneration using rodent model. Urology, 65(4), 827-832. https://doi.org/10.1016/j.urology.2004.11.024

Human embryoid body-derived stem cells in bladder regeneration using rodent model. / Frimberger, Dominic; Morales, Nelly; Shamblott, Michael; Gearhart, John D.; Gearhart, John Phillip; Lakshmanan, Yegappan.

In: Urology, Vol. 65, No. 4, 04.2005, p. 827-832.

Research output: Contribution to journalArticle

Frimberger, D, Morales, N, Shamblott, M, Gearhart, JD, Gearhart, JP & Lakshmanan, Y 2005, 'Human embryoid body-derived stem cells in bladder regeneration using rodent model', Urology, vol. 65, no. 4, pp. 827-832. https://doi.org/10.1016/j.urology.2004.11.024
Frimberger, Dominic ; Morales, Nelly ; Shamblott, Michael ; Gearhart, John D. ; Gearhart, John Phillip ; Lakshmanan, Yegappan. / Human embryoid body-derived stem cells in bladder regeneration using rodent model. In: Urology. 2005 ; Vol. 65, No. 4. pp. 827-832.
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