Nine lines of doubly cloned Simian Virus 40 transformed human fibroblasts have been isolated. Precrisis in vitro characteristics of growth rate, saturation density, T antigen expression, growth in methylcellulose suspension, protease production and infectious center formation were widely divergent, although distinct for individual lines. Lines with higher T antigen expression had higher growth rates and saturation densities but grew less well in methylcellulose. Chromosome counts in most proliferating lines were in the 38-48 range; one line had 67, whereas another, poorly growing line showed 22. No distinguishing chromosomal abnormalities were present. This biologic heterogeneity suggests distinct molecular differences underlying transformation in the lines and emphasizes the importance of considering various factors as indicative of the transformed state.
ASJC Scopus subject areas
- Cell Biology