TY - JOUR
T1 - Human cell transformation by Simian Virus 40. Biologic features of cloned lines
AU - Sack, George H.
AU - Obie, Cassandra
PY - 1981/8
Y1 - 1981/8
N2 - Nine lines of doubly cloned Simian Virus 40 transformed human fibroblasts have been isolated. Precrisis in vitro characteristics of growth rate, saturation density, T antigen expression, growth in methylcellulose suspension, protease production and infectious center formation were widely divergent, although distinct for individual lines. Lines with higher T antigen expression had higher growth rates and saturation densities but grew less well in methylcellulose. Chromosome counts in most proliferating lines were in the 38-48 range; one line had 67, whereas another, poorly growing line showed 22. No distinguishing chromosomal abnormalities were present. This biologic heterogeneity suggests distinct molecular differences underlying transformation in the lines and emphasizes the importance of considering various factors as indicative of the transformed state.
AB - Nine lines of doubly cloned Simian Virus 40 transformed human fibroblasts have been isolated. Precrisis in vitro characteristics of growth rate, saturation density, T antigen expression, growth in methylcellulose suspension, protease production and infectious center formation were widely divergent, although distinct for individual lines. Lines with higher T antigen expression had higher growth rates and saturation densities but grew less well in methylcellulose. Chromosome counts in most proliferating lines were in the 38-48 range; one line had 67, whereas another, poorly growing line showed 22. No distinguishing chromosomal abnormalities were present. This biologic heterogeneity suggests distinct molecular differences underlying transformation in the lines and emphasizes the importance of considering various factors as indicative of the transformed state.
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U2 - 10.1016/0014-4827(81)90442-0
DO - 10.1016/0014-4827(81)90442-0
M3 - Article
C2 - 6268432
AN - SCOPUS:0019816415
SN - 0014-4827
VL - 134
SP - 425
EP - 432
JO - Experimental cell research
JF - Experimental cell research
IS - 2
ER -