Human cDNA clones transcribed from an unusually high-molecular-weight RNA encode a new collagen chain

Jeanne C. Myers, Mae Jane Sun, Joseph A. D'Ippolito, Ethylin Wang Jabs, Eric G. Neilson, Arnold S. Dion

Research output: Contribution to journalArticle

Abstract

Human collagen (COL) cDNA clones were isolated from a library representing transcripts synthesized by an established rhabdomyosarcoma (RH) cell line. The 0.6-kb insert of the first isolate encodes a discontinuous collagenous sequence not homologous to type I-XVI COL chains. Sequencing of a second clone with a 4-kb insert revealed an open reading frame (ORF) of 2154 nucleotides. The deduced amino acid (aa) sequence begins with an 186-aa noncollagenous region containing seven cysteines (Cys). Several of the Cys and surrounding aa residues can be aligned with those in type XVI, XII and IX COL. Due to the presence of two long interruptions, the 524-aa collagenous region is separated into three subdomains that each have smaller interruptions of 1-6 aa. The protein terminates with an 8-aa noncollagenous peptide including an unusual single Cys which would be expected to form an interchain disulfide bond. Results of Northern blot hybridization suggest that the new COL chain may be uncommonly large since the clone identified a low-abundance RNA at least 12.4kb in size. The gene coding for RH COL is located on human chromosome 6. It is now important to elucidate the role of this unusual COL in the infrastructure of extracellular matrix.

Original languageEnglish (US)
Pages (from-to)211-217
Number of pages7
JournalGene
Volume123
Issue number2
DOIs
StatePublished - Jan 30 1993

Fingerprint

Collagen
Complementary DNA
Clone Cells
Molecular Weight
RNA
Amino Acids
Cysteine
Rhabdomyosarcoma
Collagen Type XII
Collagen Type IX
Chromosomes, Human, Pair 6
Human Chromosomes
Sequence Homology
Collagen Type I
Disulfides
Northern Blotting
Open Reading Frames
Libraries
Extracellular Matrix
Amino Acid Sequence

Keywords

  • C-terminus
  • cDNA libraries
  • gene mapping
  • noncollagenous region
  • Northern blot hybridization
  • predicted secondary structure

ASJC Scopus subject areas

  • Genetics

Cite this

Myers, J. C., Sun, M. J., D'Ippolito, J. A., Jabs, E. W., Neilson, E. G., & Dion, A. S. (1993). Human cDNA clones transcribed from an unusually high-molecular-weight RNA encode a new collagen chain. Gene, 123(2), 211-217. https://doi.org/10.1016/0378-1119(93)90126-N

Human cDNA clones transcribed from an unusually high-molecular-weight RNA encode a new collagen chain. / Myers, Jeanne C.; Sun, Mae Jane; D'Ippolito, Joseph A.; Jabs, Ethylin Wang; Neilson, Eric G.; Dion, Arnold S.

In: Gene, Vol. 123, No. 2, 30.01.1993, p. 211-217.

Research output: Contribution to journalArticle

Myers, JC, Sun, MJ, D'Ippolito, JA, Jabs, EW, Neilson, EG & Dion, AS 1993, 'Human cDNA clones transcribed from an unusually high-molecular-weight RNA encode a new collagen chain', Gene, vol. 123, no. 2, pp. 211-217. https://doi.org/10.1016/0378-1119(93)90126-N
Myers, Jeanne C. ; Sun, Mae Jane ; D'Ippolito, Joseph A. ; Jabs, Ethylin Wang ; Neilson, Eric G. ; Dion, Arnold S. / Human cDNA clones transcribed from an unusually high-molecular-weight RNA encode a new collagen chain. In: Gene. 1993 ; Vol. 123, No. 2. pp. 211-217.
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