Human cardiac high-energy phosphate metabolite concentrations by 1D-resolved NMR spectroscopy

Research output: Contribution to journalArticle

Abstract

We have developed a method that can measure high-energy phosphate metabolite concentrations in humans with 1D resolved surface-coil NMR spectroscopy. The metabolites are measured by phosphorus (31P) NMR spectroscopy, and the tissue water proton (1H) resonance from the same volume serves as an internal concentration reference. The method requires only the additional acquisition of a 1H data set, and a simple calibration, performed separately, to determine the ratio of the signal per proton to the signal per phosphorus nucleus. The quantification method is particularly useful for human cardiac spectroscopy, where it eliminates image-based tissue volumetry and the corrections for signal sensitivity and phase nonuniformity necessary in prior approaches. Corrections are introduced to account for blood and fat contributions to the spectra. The method was validated on phantoms of phosphate of varying concentrations and on the human calf muscle. In calf, the adenosine triphosphate (ATP) and phosphocreatine (PCr) concentrations were 5.6 ± 1.6 (mean ± SD) and 26 ± 4 mmol/kg wet wt, respectively. In normal heart, [ATP] was 5.8 ± 1.6 and [PCr] was 10 ± 2 mmol/kg wet wt. These values are in excellent agreement with prior NMR studies and biopsy data. The protocol is easily accommodated within existing 1D cardiac patient protocols, and the same approach is advantageous for eliminating tissue volumetry and sensitivity corrections when measuring concentrations by 2D and 3D resolved spectroscopy.

Original languageEnglish (US)
Pages (from-to)664-670
Number of pages7
JournalMagnetic Resonance in Medicine
Volume35
Issue number5
StatePublished - May 1996

Fingerprint

Magnetic Resonance Spectroscopy
Phosphates
Phosphocreatine
Phosphorus
Protons
Spectrum Analysis
Adenosine Triphosphate
Calibration
Fats
Biopsy
Muscles
Water

Keywords

  • Heart
  • Metabolite
  • Phosphorus
  • Quantification

ASJC Scopus subject areas

  • Radiology Nuclear Medicine and imaging
  • Radiological and Ultrasound Technology

Cite this

Human cardiac high-energy phosphate metabolite concentrations by 1D-resolved NMR spectroscopy. / Bottomley, Paul A; Atalar, Ergin; Weiss, Robert George.

In: Magnetic Resonance in Medicine, Vol. 35, No. 5, 05.1996, p. 664-670.

Research output: Contribution to journalArticle

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