Abstract
The objective of this study was to re-evaluate the previously published hematopoietic stem cell (HSC) expansion work using human brain endothelial cells (HUBEC). The expansion effect of contact and non-contact conditions was reported to be equivalent by others. However, we report here different results that the expansion can be achieved only with direct contact. We co-cultured human CD34+ cells with and without HUBEC contact for seven days with cytokines and the readouts were CD34+/CD38- phenotype and SCID repopulating cell (SRC) frequency. Also tested was the inhibitory effect of Wnt receptor inhibitor Dkk-1 on HUBEC contact ex vivo expansion; whether an increased expression of Wnt3 occurs on the HUBEC surface; and detection of an increased nuclear localization of beta-catenin in CD34+/CD38- cells in HUBEC contact culture condition. We conclude that the successful expansion by HUBEC contact culture is a candidate explanation based on the Wnt family protein, possibly Wnt3, expression on HUBEC.
Original language | English (US) |
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Pages (from-to) | 141-150 |
Number of pages | 10 |
Journal | Growth Factors |
Volume | 25 |
Issue number | 3 |
DOIs | |
State | Published - Jun 2007 |
Keywords
- CD34+/CD38-
- Contact culture
- Cord blood CD34+ cells
- Wnt/β-catenin
ASJC Scopus subject areas
- Endocrinology
- Clinical Biochemistry
- Cell Biology