Human brain endothelial cells (HUBEC) promote SCID repopulating cell expansion through direct contact

Xiangfei Cheng, Thomas Macvittie, Barry Meisenberg, Emily Welty, Ann Farese, Douglas Tadaki, Naoko Takebe

Research output: Contribution to journalArticlepeer-review

Abstract

The objective of this study was to re-evaluate the previously published hematopoietic stem cell (HSC) expansion work using human brain endothelial cells (HUBEC). The expansion effect of contact and non-contact conditions was reported to be equivalent by others. However, we report here different results that the expansion can be achieved only with direct contact. We co-cultured human CD34+ cells with and without HUBEC contact for seven days with cytokines and the readouts were CD34+/CD38- phenotype and SCID repopulating cell (SRC) frequency. Also tested was the inhibitory effect of Wnt receptor inhibitor Dkk-1 on HUBEC contact ex vivo expansion; whether an increased expression of Wnt3 occurs on the HUBEC surface; and detection of an increased nuclear localization of beta-catenin in CD34+/CD38- cells in HUBEC contact culture condition. We conclude that the successful expansion by HUBEC contact culture is a candidate explanation based on the Wnt family protein, possibly Wnt3, expression on HUBEC.

Original languageEnglish (US)
Pages (from-to)141-150
Number of pages10
JournalGrowth Factors
Volume25
Issue number3
DOIs
StatePublished - Jun 2007

Keywords

  • CD34+/CD38-
  • Contact culture
  • Cord blood CD34+ cells
  • Wnt/β-catenin

ASJC Scopus subject areas

  • Endocrinology
  • Clinical Biochemistry
  • Cell Biology

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