Abstract
Binding of meningitis-causing Escherichia coli K1 to human brain microvascular endothelial cells (HBMEC) contributes to traversal of the blood-brain barrier, which occurs in part by the mannose-sensitive binding of FimH. In this study, we showed that FimH also binds to HBMEC, independent of mannose, and identified ATP synthase β-subunit and actin proteins from the surface biotinylated HBMEC as the mannose-insensitive binding targets for FimH. Co-immunoprecipitation experiments in the presence of α-methyl mannose verified the binding of FimH to ATP synthase β-subunit of HBMEC. ATP synthase β-subunit antibody decreased E. coli K1 binding to HBMEC in the presence of α-methyl mannose. Taken together, these findings demonstrate that FimH of E. coli K1 binds to HBMEC in both mannose-sensitive and-insensitive manner.
Original language | English (US) |
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Pages (from-to) | 156-162 |
Number of pages | 7 |
Journal | FEMS microbiology letters |
Volume | 303 |
Issue number | 2 |
DOIs | |
State | Published - Feb 2010 |
Externally published | Yes |
Keywords
- ATP synthase β-subunit
- FimH
- HBMEC
ASJC Scopus subject areas
- Microbiology
- Molecular Biology
- Genetics