Human brain capillary endothelium: Modulation of K+ efflux and K+, Ca2+ uptake by endothelin

M. Spatz, N. Kawai, J. Bembry, F. Lenz, R. M. McCarron

Research output: Contribution to journalArticle

Abstract

This report describes K+ efflux, K+ and Ca2+ uptake responses to endothelins (ET-1 and ET-3) in cultured endothelium derived from capillaries of human brain (HBEC). ET-1 dose dependently increased K+ efflux, K+ and Ca2+ uptake in these cells. ET-1 stimulated K+ efflux occurred prior to that of K+ uptake. ET-3 was ineffective. The main contributor to the ET-1 induced K+ uptake was ouabain but not bumetanide-sensitive (Na+-K+-ATPase and Na+-K+-Cl- cotransport activity, respectively). All tested paradigms of ET-1 effects in HBEC were inhibited by selective antagonist of ET(A) but not ET(B) receptors and inhibitors of phospholipase C and receptor-operated Ca2+ channels. Activation of protein kinase C (PKC) decreased whereas inhibition of PKC increased the ET-1 stimulated K+ efflux, K+ and Ca2+ uptake in HBEC. The results indicate that ET-1 affects the HBEC ionic transport systems through activation of ET(A) receptors linked to PLC and modulated by intracellular Ca2+ mobilization and PKC.

Original languageEnglish (US)
Pages (from-to)1125-1132
Number of pages8
JournalNeurochemical Research
Volume23
Issue number8
DOIs
StatePublished - Aug 8 1998

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Keywords

  • Ca uptake
  • Endothelin-1
  • Human brain capillary endothelium
  • K efflux
  • K uptake

ASJC Scopus subject areas

  • Biochemistry
  • Cellular and Molecular Neuroscience

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