Human beta defensin is absent in burn blister fluid

M. R. Ortega, T. Ganz, S. M. Milner

Research output: Contribution to journalArticle

Abstract

Defensins are a family of cationic antimicrobial peptides that participate in innate host defence. Human beta defensin-2 (HBD-2) is produced by human keratinocytes, and has a potent bactericidal activity against a wide spectrum of microorganisms. We have recently shown that expression of HBD-2 is present in normal skin and lost in the full-thickness burn wound. Defensins have been found in the blister fluid of chronic wounds. Our study was designed to examine blister fluid from partial-thickness burns for defensin content. Fluid from five patients was collected from partial-thickness burn blisters, and then analysed by sandwich Enzyme-Linked Immunosorbent Assay (ELISA) with a monoclonal antibody and rabbit polyclonal antibody to HBD-2. The assay was validated against a Western blot assay for HBD-2 in samples of bronchoalveolar lavage fluid from patients with inflammatory lung disease. No HBD-2 was detectable in any of the burn blister fluids analysed. HBD-2 is lost in the full-thickness burn wound, and we have now demonstrated its absence in burn blister fluid. This finding represents evidence of a host defence defect within the burn wound and suggests a possible therapeutic role for antimicrobial peptides in the management of burn wounds. Copyright (C) 2000 Elsevier Science Ltd and ISBI.

Original languageEnglish (US)
Pages (from-to)724-726
Number of pages3
JournalBurns
Volume26
Issue number8
DOIs
StatePublished - Dec 2000
Externally publishedYes

Fingerprint

beta-Defensins
Blister
Defensins
Wounds and Injuries
Antimicrobial Cationic Peptides
Bronchoalveolar Lavage Fluid
Burns
Keratinocytes
Lung Diseases
human DEFB4A protein
Western Blotting
Enzyme-Linked Immunosorbent Assay
Monoclonal Antibodies
Rabbits
Skin
Peptides
Antibodies

Keywords

  • Antimicrobial peptides
  • Blister fluid
  • Burns
  • Defensins
  • Sepsis

ASJC Scopus subject areas

  • Emergency Medicine
  • Surgery

Cite this

Ortega, M. R., Ganz, T., & Milner, S. M. (2000). Human beta defensin is absent in burn blister fluid. Burns, 26(8), 724-726. https://doi.org/10.1016/S0305-4179(00)00052-8

Human beta defensin is absent in burn blister fluid. / Ortega, M. R.; Ganz, T.; Milner, S. M.

In: Burns, Vol. 26, No. 8, 12.2000, p. 724-726.

Research output: Contribution to journalArticle

Ortega, MR, Ganz, T & Milner, SM 2000, 'Human beta defensin is absent in burn blister fluid', Burns, vol. 26, no. 8, pp. 724-726. https://doi.org/10.1016/S0305-4179(00)00052-8
Ortega, M. R. ; Ganz, T. ; Milner, S. M. / Human beta defensin is absent in burn blister fluid. In: Burns. 2000 ; Vol. 26, No. 8. pp. 724-726.
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