Human basophils secrete IL-3: Evidence of autocrine priming for phenotypic and functional responses in allergic disease

John Thomas Schroeder, Kristin L. Chichester, Anja P. Bieneman

Research output: Contribution to journalArticle

Abstract

Although IL-3 is commonly recognized for its growth factor-like activity, in vitro studies have long demonstrated a unique capacity for this cytokine to also augment the proinflammatory properties and phenotype of human basophils. In particular, basophils secrete mediators that are hallmarks in allergic disease, including vasoactive amines (e.g., histamine), lipid metabolites (e.g., leukotriene C4), and cytokines (e.g., IL-4/IL-13), which are all markedly enhanced with IL-3 pretreatment. This priming phenomenon is observed in response to both IgE-dependent and IgE-independent stimulation. Additionally, IL-3 directly activates basophils for IL-13 secretion and enhanced CD69 expression, two markers that are elevated in allergic subjects. Lymphocytes are commonly thought to be the source of the IL-3 that primes for these basophil responses. However, we demonstrate herein for the first time that basophils themselves rapidly produce IL-3 (within 4 h) in response to IgE-dependent activation. More importantly, our findings definitively show that basophils rapidly bind and utilize the IL-3 they produce, as evidenced by functional and phenotypic activity that is inhibited in the presence of neutralizing anti-IL-3 receptor (CD123) Abs. We predict that autocrine IL-3 activity resulting from low-level IgE/FcεRI cross-linking by specific allergen represents an important mechanism behind the hyperreactive nature of basophils that has long been observed in allergic disease.

Original languageEnglish (US)
Pages (from-to)2432-2438
Number of pages7
JournalJournal of Immunology
Volume182
Issue number4
DOIs
StatePublished - Feb 15 2009

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Basophils
Interleukin-3
Immunoglobulin E
Interleukin-13
Interleukin-3 Receptors
Cytokines
Leukotriene C4
Interleukin-4
Allergens
Histamine
Amines
Intercellular Signaling Peptides and Proteins
Lymphocytes
Phenotype
Lipids

ASJC Scopus subject areas

  • Immunology
  • Medicine(all)

Cite this

Human basophils secrete IL-3 : Evidence of autocrine priming for phenotypic and functional responses in allergic disease. / Schroeder, John Thomas; Chichester, Kristin L.; Bieneman, Anja P.

In: Journal of Immunology, Vol. 182, No. 4, 15.02.2009, p. 2432-2438.

Research output: Contribution to journalArticle

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