Human autoantibodies reveal titin as a chromosomal protein

Cristina Machado, Claudio E. Sunkel, Deborah J. Andrew

Research output: Contribution to journalArticlepeer-review

103 Scopus citations


Assembly of the higher-order structure of mitotic chromosomes is a prerequisite for proper chromosome condensations segregation and integrity. Understanding the details of this process has been limited because very few proteins involved in the assembly of chromosome structure have been discovered. Using a human autoimmune scleroderma serum that identifies a chromosomal protein in human cells and Drosophila embryos, we cloned the corresponding Drosophila gene that encodes the homologue of vertebrate titin based on protein size, sequence similarity, developmental expression and subcellular localization. Titin is a giant sarcomeric protein responsible for the elasticity of striated muscle that may also function as a molecular scaffold for myofibrillar assembly. Molecular analysis and immunostaining with antibodies to multiple titin epitopes indicates that the chromosomal and muscle forms of titin may vary in their NH2 termini. The identification of titin as a chromosomal component provides a molecular basis for chromosome structure and elasticity.

Original languageEnglish (US)
Pages (from-to)321-333
Number of pages13
JournalJournal of Cell Biology
Issue number2
StatePublished - Apr 20 1998

ASJC Scopus subject areas

  • Cell Biology


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