Human Aurora-B binds to a proteasome α-subunit HC8 and undergoes degradation in a proteasome-dependent manner

Fengjue Shu, Shuguang Guo, Yongjun Dang, Meiyan Qi, Guangjin Zhou, Zekun Guo, Ying Zhang, Chaoqun Wu, Shouyuan Zhao, Long Yu

Research output: Contribution to journalArticlepeer-review

Abstract

Human Aurora/Ip11-related kinase 2 (Aurora-B) is a key regulator of mitosis. Here human proteasome α-subunit C8 (HC8) was identified to interact with the Aurora-B by yeast two-hybrid screen. This finding was confirmed by GST pull-down assays and immunoprecipitation experiments. The Aurora-B protein level increased in HeLa cells cultured with proteasome inhibitor ALLN. Our data suggest that Aurora-B might undergo degradation by binding to HC8 in a proteasome-dependent manner during mitosis.

Original languageEnglish (US)
Pages (from-to)157-162
Number of pages6
JournalMolecular and Cellular Biochemistry
Volume254
Issue number1-2
DOIs
StatePublished - Dec 1 2003

Keywords

  • Aurora-B
  • Degradation
  • HC8
  • Mitosis
  • Proteasome

ASJC Scopus subject areas

  • Molecular Biology
  • Clinical Biochemistry
  • Cell Biology

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