Human Aurora-B binds to a proteasome α-subunit HC8 and undergoes degradation in a proteasome-dependent manner

Fengjue Shu, Shuguang Guo, Yongjun Dang, Meiyan Qi, Guangjin Zhou, Zekun Guo, Ying Zhang, Chaoqun Wu, Shouyuan Zhao, Long Yu

Research output: Contribution to journalArticle

Abstract

Human Aurora/Ip11-related kinase 2 (Aurora-B) is a key regulator of mitosis. Here human proteasome α-subunit C8 (HC8) was identified to interact with the Aurora-B by yeast two-hybrid screen. This finding was confirmed by GST pull-down assays and immunoprecipitation experiments. The Aurora-B protein level increased in HeLa cells cultured with proteasome inhibitor ALLN. Our data suggest that Aurora-B might undergo degradation by binding to HC8 in a proteasome-dependent manner during mitosis.

Original languageEnglish (US)
Pages (from-to)157-162
Number of pages6
JournalMolecular and Cellular Biochemistry
Volume254
Issue number1-2
DOIs
StatePublished - Dec 2003
Externally publishedYes

Fingerprint

Proteasome Endopeptidase Complex
Mitosis
Degradation
Proteasome Inhibitors
HeLa Cells
Immunoprecipitation
Yeast
Assays
Yeasts
Experiments
human AURKB protein
IgA receptor

Keywords

  • Aurora-B
  • Degradation
  • HC8
  • Mitosis
  • Proteasome

ASJC Scopus subject areas

  • Clinical Biochemistry
  • Molecular Biology
  • Genetics
  • Cell Biology

Cite this

Human Aurora-B binds to a proteasome α-subunit HC8 and undergoes degradation in a proteasome-dependent manner. / Shu, Fengjue; Guo, Shuguang; Dang, Yongjun; Qi, Meiyan; Zhou, Guangjin; Guo, Zekun; Zhang, Ying; Wu, Chaoqun; Zhao, Shouyuan; Yu, Long.

In: Molecular and Cellular Biochemistry, Vol. 254, No. 1-2, 12.2003, p. 157-162.

Research output: Contribution to journalArticle

Shu, F, Guo, S, Dang, Y, Qi, M, Zhou, G, Guo, Z, Zhang, Y, Wu, C, Zhao, S & Yu, L 2003, 'Human Aurora-B binds to a proteasome α-subunit HC8 and undergoes degradation in a proteasome-dependent manner', Molecular and Cellular Biochemistry, vol. 254, no. 1-2, pp. 157-162. https://doi.org/10.1023/A:1027317014159
Shu, Fengjue ; Guo, Shuguang ; Dang, Yongjun ; Qi, Meiyan ; Zhou, Guangjin ; Guo, Zekun ; Zhang, Ying ; Wu, Chaoqun ; Zhao, Shouyuan ; Yu, Long. / Human Aurora-B binds to a proteasome α-subunit HC8 and undergoes degradation in a proteasome-dependent manner. In: Molecular and Cellular Biochemistry. 2003 ; Vol. 254, No. 1-2. pp. 157-162.
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