Human AML cells in NOD/SCID mice: Engraftment potential and gene expression

R. Lumkul, N. C. Gorin, M. T. Malehorn, G. T. Hoehn, R. Zheng, B. Baldwin, D. Small, S. Gore, D. Smith, P. S. Meltzer, C. I. Civin

Research output: Contribution to journalArticlepeer-review

Abstract

Most cases of human acute myeloid leukemia (AML) engraft in irradiated non-obese diabetic/severe combined immunodeficient (NOD/SCID) mice. Intravenous transfer of as few as 105 human AML cells resulted in engraftment. Cases with poor prognosis clinical features, including FLT3 mutations, tended to engraft efficiently. Nevertheless, AML cells obtained from patients at relapse did not engraft more efficiently than cells obtained from the same patients at initial diagnosis. One passage of human AML cells in NOD/SCID mice did not appear to select for increased virulence, as measured by serial transplantation efficiency. Finally, cDNA microarray analyses indicated that ∼95% of genes were expressed at similar levels in human AML cells immunopurified after growth in mice, as compared to cells assessed directly from patients. Thus, the growth of human AML cells in NOD/SCID mice could yield large numbers of human AML cells for direct experimental use and could also function as a renewable, potentially unlimited source of leukemia cells, via serial transplantation.

Original languageEnglish (US)
Pages (from-to)1818-1826
Number of pages9
JournalLeukemia
Volume16
Issue number9
DOIs
StatePublished - 2002

Keywords

  • Acute myeloid leukaemia
  • Flow cytometry
  • Gene expression
  • NOD/SCID mice
  • cDNA microarrays

ASJC Scopus subject areas

  • Hematology
  • Oncology
  • Cancer Research

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