Human aflatoxin albumin adducts quantitatively compared by ELISA, HPLC with fluorescence detection, and HPLC with isotope dilution mass spectrometry

Leslie F. McCoy, Peter F. Scholl, Anne E. Sutcliffe, Stephanie M. Kieszak, Carissa D. Powers, Helen S. Rogers, Yun Yun Gong, John Davis Groopman, Christopher P. Wild, Rosemary L. Schleicher

Research output: Contribution to journalArticle

Abstract

Essential to the conduct of epidemiologic studies examining aflatoxin exposure and the risk of heptocellular carcinoma, impaired growth, and acute toxicity has been the development of quantitative biomarkers of exposure to aflatoxins, particularly aflatoxin B1. In this study, identical serum sample sets were analyzed for aflatoxin-albumin adducts by ELISA, high-performance liquid chromatography (HPLC) with fluorescence detection (HPLC-f), and HPLC with isotope dilution mass spectrometry (IDMS). The human samples analyzed were from an acute aflatoxicosis outbreak in Kenya in 2004 (n = 102) and the measured values ranged from 0.018 to 67.0, nondetectable to 13.6, and 0.002 to 17.7 ng/mg albumin for the respective methods. The Deming regression slopes for the HPLC-f and ELISA concentrations as a function of the IDMS concentrations were 0.71 (r2 = 0.95) and 3.3 (r2 = 0.96), respectively. When the samples were classified as cases or controls, based on clinical diagnosis, all methods were predictive of outcome (P <0.01). Further, to evaluate assay precision, duplicate samples were prepared at three levels by dilution of an exposed human sample and were analyzed on three separate days. Excluding one assay value by ELISA and one assay by HPLC-f, the overall relative SD were 8.7%, 10.5%, and 9.4% for IDMS, HPLC-f, and ELISA, respectively. IDMS was the most sensitive technique and HPLC-f was the least sensitive method. Overall, this study shows an excellent correlation between three independent methodologies conducted in different laboratories and supports the validation of these technologies for assessment of human exposure to this environmental toxin and carcinogen.

Original languageEnglish (US)
Pages (from-to)1653-1657
Number of pages5
JournalCancer Epidemiology Biomarkers and Prevention
Volume17
Issue number7
DOIs
StatePublished - Jul 2008

Fingerprint

Isotopes
Mass Spectrometry
Fluorescence
Enzyme-Linked Immunosorbent Assay
High Pressure Liquid Chromatography
Aflatoxins
Environmental Carcinogens
Biomedical Technology Assessment
Aflatoxin B1
Kenya
aflatoxin-albumin adduct
Disease Outbreaks
Epidemiologic Studies
Albumins
Biomarkers
Carcinoma
Growth
Serum

ASJC Scopus subject areas

  • Epidemiology
  • Oncology

Cite this

Human aflatoxin albumin adducts quantitatively compared by ELISA, HPLC with fluorescence detection, and HPLC with isotope dilution mass spectrometry. / McCoy, Leslie F.; Scholl, Peter F.; Sutcliffe, Anne E.; Kieszak, Stephanie M.; Powers, Carissa D.; Rogers, Helen S.; Gong, Yun Yun; Groopman, John Davis; Wild, Christopher P.; Schleicher, Rosemary L.

In: Cancer Epidemiology Biomarkers and Prevention, Vol. 17, No. 7, 07.2008, p. 1653-1657.

Research output: Contribution to journalArticle

McCoy, Leslie F. ; Scholl, Peter F. ; Sutcliffe, Anne E. ; Kieszak, Stephanie M. ; Powers, Carissa D. ; Rogers, Helen S. ; Gong, Yun Yun ; Groopman, John Davis ; Wild, Christopher P. ; Schleicher, Rosemary L. / Human aflatoxin albumin adducts quantitatively compared by ELISA, HPLC with fluorescence detection, and HPLC with isotope dilution mass spectrometry. In: Cancer Epidemiology Biomarkers and Prevention. 2008 ; Vol. 17, No. 7. pp. 1653-1657.
@article{49f5242e4fb94b3e928c2c0681436601,
title = "Human aflatoxin albumin adducts quantitatively compared by ELISA, HPLC with fluorescence detection, and HPLC with isotope dilution mass spectrometry",
abstract = "Essential to the conduct of epidemiologic studies examining aflatoxin exposure and the risk of heptocellular carcinoma, impaired growth, and acute toxicity has been the development of quantitative biomarkers of exposure to aflatoxins, particularly aflatoxin B1. In this study, identical serum sample sets were analyzed for aflatoxin-albumin adducts by ELISA, high-performance liquid chromatography (HPLC) with fluorescence detection (HPLC-f), and HPLC with isotope dilution mass spectrometry (IDMS). The human samples analyzed were from an acute aflatoxicosis outbreak in Kenya in 2004 (n = 102) and the measured values ranged from 0.018 to 67.0, nondetectable to 13.6, and 0.002 to 17.7 ng/mg albumin for the respective methods. The Deming regression slopes for the HPLC-f and ELISA concentrations as a function of the IDMS concentrations were 0.71 (r2 = 0.95) and 3.3 (r2 = 0.96), respectively. When the samples were classified as cases or controls, based on clinical diagnosis, all methods were predictive of outcome (P <0.01). Further, to evaluate assay precision, duplicate samples were prepared at three levels by dilution of an exposed human sample and were analyzed on three separate days. Excluding one assay value by ELISA and one assay by HPLC-f, the overall relative SD were 8.7{\%}, 10.5{\%}, and 9.4{\%} for IDMS, HPLC-f, and ELISA, respectively. IDMS was the most sensitive technique and HPLC-f was the least sensitive method. Overall, this study shows an excellent correlation between three independent methodologies conducted in different laboratories and supports the validation of these technologies for assessment of human exposure to this environmental toxin and carcinogen.",
author = "McCoy, {Leslie F.} and Scholl, {Peter F.} and Sutcliffe, {Anne E.} and Kieszak, {Stephanie M.} and Powers, {Carissa D.} and Rogers, {Helen S.} and Gong, {Yun Yun} and Groopman, {John Davis} and Wild, {Christopher P.} and Schleicher, {Rosemary L.}",
year = "2008",
month = "7",
doi = "10.1158/1055-9965.EPI-07-2780",
language = "English (US)",
volume = "17",
pages = "1653--1657",
journal = "Cancer Epidemiology Biomarkers and Prevention",
issn = "1055-9965",
publisher = "American Association for Cancer Research Inc.",
number = "7",

}

TY - JOUR

T1 - Human aflatoxin albumin adducts quantitatively compared by ELISA, HPLC with fluorescence detection, and HPLC with isotope dilution mass spectrometry

AU - McCoy, Leslie F.

AU - Scholl, Peter F.

AU - Sutcliffe, Anne E.

AU - Kieszak, Stephanie M.

AU - Powers, Carissa D.

AU - Rogers, Helen S.

AU - Gong, Yun Yun

AU - Groopman, John Davis

AU - Wild, Christopher P.

AU - Schleicher, Rosemary L.

PY - 2008/7

Y1 - 2008/7

N2 - Essential to the conduct of epidemiologic studies examining aflatoxin exposure and the risk of heptocellular carcinoma, impaired growth, and acute toxicity has been the development of quantitative biomarkers of exposure to aflatoxins, particularly aflatoxin B1. In this study, identical serum sample sets were analyzed for aflatoxin-albumin adducts by ELISA, high-performance liquid chromatography (HPLC) with fluorescence detection (HPLC-f), and HPLC with isotope dilution mass spectrometry (IDMS). The human samples analyzed were from an acute aflatoxicosis outbreak in Kenya in 2004 (n = 102) and the measured values ranged from 0.018 to 67.0, nondetectable to 13.6, and 0.002 to 17.7 ng/mg albumin for the respective methods. The Deming regression slopes for the HPLC-f and ELISA concentrations as a function of the IDMS concentrations were 0.71 (r2 = 0.95) and 3.3 (r2 = 0.96), respectively. When the samples were classified as cases or controls, based on clinical diagnosis, all methods were predictive of outcome (P <0.01). Further, to evaluate assay precision, duplicate samples were prepared at three levels by dilution of an exposed human sample and were analyzed on three separate days. Excluding one assay value by ELISA and one assay by HPLC-f, the overall relative SD were 8.7%, 10.5%, and 9.4% for IDMS, HPLC-f, and ELISA, respectively. IDMS was the most sensitive technique and HPLC-f was the least sensitive method. Overall, this study shows an excellent correlation between three independent methodologies conducted in different laboratories and supports the validation of these technologies for assessment of human exposure to this environmental toxin and carcinogen.

AB - Essential to the conduct of epidemiologic studies examining aflatoxin exposure and the risk of heptocellular carcinoma, impaired growth, and acute toxicity has been the development of quantitative biomarkers of exposure to aflatoxins, particularly aflatoxin B1. In this study, identical serum sample sets were analyzed for aflatoxin-albumin adducts by ELISA, high-performance liquid chromatography (HPLC) with fluorescence detection (HPLC-f), and HPLC with isotope dilution mass spectrometry (IDMS). The human samples analyzed were from an acute aflatoxicosis outbreak in Kenya in 2004 (n = 102) and the measured values ranged from 0.018 to 67.0, nondetectable to 13.6, and 0.002 to 17.7 ng/mg albumin for the respective methods. The Deming regression slopes for the HPLC-f and ELISA concentrations as a function of the IDMS concentrations were 0.71 (r2 = 0.95) and 3.3 (r2 = 0.96), respectively. When the samples were classified as cases or controls, based on clinical diagnosis, all methods were predictive of outcome (P <0.01). Further, to evaluate assay precision, duplicate samples were prepared at three levels by dilution of an exposed human sample and were analyzed on three separate days. Excluding one assay value by ELISA and one assay by HPLC-f, the overall relative SD were 8.7%, 10.5%, and 9.4% for IDMS, HPLC-f, and ELISA, respectively. IDMS was the most sensitive technique and HPLC-f was the least sensitive method. Overall, this study shows an excellent correlation between three independent methodologies conducted in different laboratories and supports the validation of these technologies for assessment of human exposure to this environmental toxin and carcinogen.

UR - http://www.scopus.com/inward/record.url?scp=53549115938&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=53549115938&partnerID=8YFLogxK

U2 - 10.1158/1055-9965.EPI-07-2780

DO - 10.1158/1055-9965.EPI-07-2780

M3 - Article

C2 - 18628416

AN - SCOPUS:53549115938

VL - 17

SP - 1653

EP - 1657

JO - Cancer Epidemiology Biomarkers and Prevention

JF - Cancer Epidemiology Biomarkers and Prevention

SN - 1055-9965

IS - 7

ER -