TY - JOUR
T1 - Human adipose stromal vascular cell delivery in a fibrin spray
AU - Zimmerlin, Ludovic
AU - Rubin, J. Peter
AU - Pfeifer, Melanie E.
AU - Moore, Linda R.
AU - Donnenberg, Vera S.
AU - Donnenberg, Albert D.
N1 - Funding Information:
This project was supported by Production Assistance for Cellular Therapy (PACT) under contract No. N01-HB-37165 and No. R01-HL-085819 from the National Heart, Lung, and Blood Institute and by R01 CA 114246-7 from the National Cancer Institute .
PY - 2013/1
Y1 - 2013/1
N2 - Background aims. Adipose tissue represents a practical source of autologous mesenchymal stromal cells (MSCs) and vascular-endothelial progenitor cells, available for regenerative therapy without in vitro expansion. One of the problems confronting the therapeutic application of such cells is how to immobilize them at the wound site. We evaluated in vitro the growth and differentiation of human adipose stromal vascular fraction (SVF) cells after delivery through the use of a fibrin spray system. Methods. SVF cells were harvested from four human adult patients undergoing elective abdominoplasty, through the use of the LipiVage system. After collagenase digestion, mesenchymal and endothelial progenitor cells (pericytes, supra-adventitial stromal cells, endothelial progenitors) were quantified by flow cytometry before culture. SVF cells were applied to culture vessels by means of the Tisseel fibrin spray system. SVF cell growth and differentiation were documented by immunofluorescence staining and photomicrography. Results. SVF cells remained viable after application and were expanded up to 3 weeks, when they reached confluence and adipogenic differentiation. Under angiogenic conditions, SVF cells formed endothelial (vWF+, CD31+ and CD34+) tubules surrounded by CD146+ and α-smooth muscle actin+ perivascular/stromal cells. Conclusions. Human adipose tissue is a rich source of autologous stem cells, which are readily available for regenerative applications such as wound healing, without in vitro expansion. Our results indicate that mesenchymal and endothelial progenitor cells, prepared in a closed system from unpassaged lipoaspirate samples, retain their growth and differentiation capacity when applied and immobilized on a substrate using a clinically approved fibrin sealant spray system.
AB - Background aims. Adipose tissue represents a practical source of autologous mesenchymal stromal cells (MSCs) and vascular-endothelial progenitor cells, available for regenerative therapy without in vitro expansion. One of the problems confronting the therapeutic application of such cells is how to immobilize them at the wound site. We evaluated in vitro the growth and differentiation of human adipose stromal vascular fraction (SVF) cells after delivery through the use of a fibrin spray system. Methods. SVF cells were harvested from four human adult patients undergoing elective abdominoplasty, through the use of the LipiVage system. After collagenase digestion, mesenchymal and endothelial progenitor cells (pericytes, supra-adventitial stromal cells, endothelial progenitors) were quantified by flow cytometry before culture. SVF cells were applied to culture vessels by means of the Tisseel fibrin spray system. SVF cell growth and differentiation were documented by immunofluorescence staining and photomicrography. Results. SVF cells remained viable after application and were expanded up to 3 weeks, when they reached confluence and adipogenic differentiation. Under angiogenic conditions, SVF cells formed endothelial (vWF+, CD31+ and CD34+) tubules surrounded by CD146+ and α-smooth muscle actin+ perivascular/stromal cells. Conclusions. Human adipose tissue is a rich source of autologous stem cells, which are readily available for regenerative applications such as wound healing, without in vitro expansion. Our results indicate that mesenchymal and endothelial progenitor cells, prepared in a closed system from unpassaged lipoaspirate samples, retain their growth and differentiation capacity when applied and immobilized on a substrate using a clinically approved fibrin sealant spray system.
KW - Adipose-derived stem cells
KW - Multipotent mesenchymal stromal cells
KW - Pericytes
KW - Perivascular cells
KW - Regenerative therapy
KW - Supra-adventitial adipose stromal cells
KW - Wound healing
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U2 - 10.1016/j.jcyt.2012.10.009
DO - 10.1016/j.jcyt.2012.10.009
M3 - Article
C2 - 23260090
AN - SCOPUS:84875420721
SN - 1465-3249
VL - 15
SP - 102
EP - 108
JO - Cytotherapy
JF - Cytotherapy
IS - 1
ER -