Human β-defensin 2 is expressed and associated with Mycobacterium tuberculosis during infection of human alveolar epithelial cells

Bruno Rivas-Santiago, Stephan K. Schwander, Carmen Sarabia, Gill Diamond, Marcia E. Klein-Patel, Rogelio Hernandez-Pando, Jerrold J. Ellner, Eduardo Sada

Research output: Contribution to journalArticle

Abstract

To determine the role of human β-defensin 2 (HBD-2) in human tuberculosis, we studied the in vitro induction of HBD-2 gene expression by Mycobacterium tuberculosis H37Rv infection in the human lung epithelial cell line A549, in alveolar macrophages (AM), and in blood monocytes (MN) by reverse transcription-PCR. We also studied the induction of HBD-2 gene expression by mannose lipoarabinomannan (manLAM) from M. tuberculosis. Intracellular production of HBD-2 peptide was detected by immunocytochemistry and electron microscopy. Our results demonstrated that there was induction of HBD-2 mRNA in A549 cells after infection with M. tuberculosis at various multiplicities of infection (MOI) and that there was stimulation with manLAM. AM expressed the HBD-2 gene only at a high MOI with M. tuberculosis. MN did not express HBD-2 at any of the experimental M. tuberculosis MOI. Immunostaining revealed the presence of intracellular HBD-2 peptide in A549 cells following infection with M. tuberculosis, and the staining was more intense in areas where there were M. tuberculosis clusters. By using electron microscopy we also demonstrated production of HBD-2 after M. tuberculosis infection and adherence of HBD-2 to the membranes of M. tuberculosis. Alveolar epithelial cells are among the first cells to encounter M. tuberculosis following aerogenic infection. As HBD-2 has been shown to control growth of M. tuberculosis and has chemotactic activity, our results suggest that HBD-2 induction by M. tuberculosis may have a role in the pathogenesis of human tuberculosis.

Original languageEnglish (US)
Pages (from-to)4505-4511
Number of pages7
JournalInfection and Immunity
Volume73
Issue number8
DOIs
StatePublished - Aug 2005
Externally publishedYes

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Defensins
Alveolar Epithelial Cells
Mycobacterium tuberculosis
Infection
Alveolar Macrophages
Mannose
Monocytes
Electron Microscopy
Tuberculosis
Gene Expression
Peptides
Mycobacterium Infections
Reverse Transcription

ASJC Scopus subject areas

  • Immunology

Cite this

Rivas-Santiago, B., Schwander, S. K., Sarabia, C., Diamond, G., Klein-Patel, M. E., Hernandez-Pando, R., ... Sada, E. (2005). Human β-defensin 2 is expressed and associated with Mycobacterium tuberculosis during infection of human alveolar epithelial cells. Infection and Immunity, 73(8), 4505-4511. https://doi.org/10.1128/IAI.73.8.4505-4511.2005

Human β-defensin 2 is expressed and associated with Mycobacterium tuberculosis during infection of human alveolar epithelial cells. / Rivas-Santiago, Bruno; Schwander, Stephan K.; Sarabia, Carmen; Diamond, Gill; Klein-Patel, Marcia E.; Hernandez-Pando, Rogelio; Ellner, Jerrold J.; Sada, Eduardo.

In: Infection and Immunity, Vol. 73, No. 8, 08.2005, p. 4505-4511.

Research output: Contribution to journalArticle

Rivas-Santiago, B, Schwander, SK, Sarabia, C, Diamond, G, Klein-Patel, ME, Hernandez-Pando, R, Ellner, JJ & Sada, E 2005, 'Human β-defensin 2 is expressed and associated with Mycobacterium tuberculosis during infection of human alveolar epithelial cells', Infection and Immunity, vol. 73, no. 8, pp. 4505-4511. https://doi.org/10.1128/IAI.73.8.4505-4511.2005
Rivas-Santiago, Bruno ; Schwander, Stephan K. ; Sarabia, Carmen ; Diamond, Gill ; Klein-Patel, Marcia E. ; Hernandez-Pando, Rogelio ; Ellner, Jerrold J. ; Sada, Eduardo. / Human β-defensin 2 is expressed and associated with Mycobacterium tuberculosis during infection of human alveolar epithelial cells. In: Infection and Immunity. 2005 ; Vol. 73, No. 8. pp. 4505-4511.
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