Human μ opiate receptor. cDNA and genomic clones, pharmacologic characterization and chromosomal assignment

Jia Bei Wang; Peter S. Johnson; Antonio M. Persico; Anita L. Hawkins; Constance A. Griffin; George R. Uhl

doi:10.1016/0014-5793(94)80368-4

Human μ opiate receptor. cDNA and genomic clones, pharmacologic characterization and chromosomal assignment

Jia Bei Wang, Peter S. Johnson, Antonio M. Persico, Anita L. Hawkins, Constance A. Griffin, George R. Uhl

School of Medicine

Research output: Contribution to journal › Article › peer-review

281 Scopus citations

Abstract

A human μ opiate receptor cDNA has been identified from a cerebral cortical cDNA library using sequences from the rat μ opiate receptor cDNA. The human μ opiate receptor (hμOR1) shares 95% amino acid identity with the rat sequence. The expressed μOR1 recognizes tested opiate drugs and opioid peptides in a sodium- and GTP-sensitive fashion with affinities virtually identical to those displayed by the rat μ opiate receptor. Effects on cyclic AMP are similar to those noted for the rat μ opiate receptor. An 18 kb genomic clone hybridizing with the hμOR1 cDNA contains 63 and 489 bp exonic sequences flanked by splice donor/acceptor sequences. Analysis of hybridization to DNA prepared from human rodent hybrid cell lines and chromosomal in situ hybridization studies indicate localization to 6q24-25. An MspI polymorphism, producing a 3.7 kb band, may prove useful in assessing this gene's involvement in neuropsychiatric disorders involving opiatergic systems.

Original language	English (US)
Pages (from-to)	217-222
Number of pages	6
Journal	FEBS Letters
Volume	338
Issue number	2
DOIs	https://doi.org/10.1016/0014-5793(94)80368-4
State	Published - Jan 31 1994

Keywords

Addiction
Enkephalin
G-linked receptor
Heroin
Morphine
Pain

ASJC Scopus subject areas

Biochemistry
Biophysics
Molecular Biology

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10.1016/0014-5793(94)80368-4

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title = "Human μ opiate receptor. cDNA and genomic clones, pharmacologic characterization and chromosomal assignment",

abstract = "A human μ opiate receptor cDNA has been identified from a cerebral cortical cDNA library using sequences from the rat μ opiate receptor cDNA. The human μ opiate receptor (hμOR1) shares 95% amino acid identity with the rat sequence. The expressed μOR1 recognizes tested opiate drugs and opioid peptides in a sodium- and GTP-sensitive fashion with affinities virtually identical to those displayed by the rat μ opiate receptor. Effects on cyclic AMP are similar to those noted for the rat μ opiate receptor. An 18 kb genomic clone hybridizing with the hμOR1 cDNA contains 63 and 489 bp exonic sequences flanked by splice donor/acceptor sequences. Analysis of hybridization to DNA prepared from human rodent hybrid cell lines and chromosomal in situ hybridization studies indicate localization to 6q24-25. An MspI polymorphism, producing a 3.7 kb band, may prove useful in assessing this gene's involvement in neuropsychiatric disorders involving opiatergic systems.",

keywords = "Addiction, Enkephalin, G-linked receptor, Heroin, Morphine, Pain",

author = "Wang, {Jia Bei} and Johnson, {Peter S.} and Persico, {Antonio M.} and Hawkins, {Anita L.} and Griffin, {Constance A.} and Uhl, {George R.}",

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doi = "10.1016/0014-5793(94)80368-4",

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T1 - Human μ opiate receptor. cDNA and genomic clones, pharmacologic characterization and chromosomal assignment

AU - Wang, Jia Bei

AU - Johnson, Peter S.

AU - Persico, Antonio M.

AU - Hawkins, Anita L.

AU - Griffin, Constance A.

AU - Uhl, George R.

PY - 1994/1/31

Y1 - 1994/1/31

N2 - A human μ opiate receptor cDNA has been identified from a cerebral cortical cDNA library using sequences from the rat μ opiate receptor cDNA. The human μ opiate receptor (hμOR1) shares 95% amino acid identity with the rat sequence. The expressed μOR1 recognizes tested opiate drugs and opioid peptides in a sodium- and GTP-sensitive fashion with affinities virtually identical to those displayed by the rat μ opiate receptor. Effects on cyclic AMP are similar to those noted for the rat μ opiate receptor. An 18 kb genomic clone hybridizing with the hμOR1 cDNA contains 63 and 489 bp exonic sequences flanked by splice donor/acceptor sequences. Analysis of hybridization to DNA prepared from human rodent hybrid cell lines and chromosomal in situ hybridization studies indicate localization to 6q24-25. An MspI polymorphism, producing a 3.7 kb band, may prove useful in assessing this gene's involvement in neuropsychiatric disorders involving opiatergic systems.

AB - A human μ opiate receptor cDNA has been identified from a cerebral cortical cDNA library using sequences from the rat μ opiate receptor cDNA. The human μ opiate receptor (hμOR1) shares 95% amino acid identity with the rat sequence. The expressed μOR1 recognizes tested opiate drugs and opioid peptides in a sodium- and GTP-sensitive fashion with affinities virtually identical to those displayed by the rat μ opiate receptor. Effects on cyclic AMP are similar to those noted for the rat μ opiate receptor. An 18 kb genomic clone hybridizing with the hμOR1 cDNA contains 63 and 489 bp exonic sequences flanked by splice donor/acceptor sequences. Analysis of hybridization to DNA prepared from human rodent hybrid cell lines and chromosomal in situ hybridization studies indicate localization to 6q24-25. An MspI polymorphism, producing a 3.7 kb band, may prove useful in assessing this gene's involvement in neuropsychiatric disorders involving opiatergic systems.

KW - Addiction

KW - Enkephalin

KW - G-linked receptor

KW - Heroin

KW - Morphine

KW - Pain

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Human μ opiate receptor. cDNA and genomic clones, pharmacologic characterization and chromosomal assignment

Abstract

Keywords

ASJC Scopus subject areas

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