TY - JOUR
T1 - H+/e- stoichiometry for NADH dehydrogenase I and dimethyl sulfoxide reductase in anaerobically grown Escherichia coli cells
AU - Bogachev, Alexander V.
AU - Murtazina, Rakhilya A.
AU - Skulachev, Vladimir P.
PY - 1996
Y1 - 1996
N2 - Anaerobically grown Escherichia coli cells were shown to acidify the reaction medium in response to oxygen or dimethyl sulfoxide (DMSO) pulses, with the H+/e- stoichiometry being close to 2.5 and 1.5, respectively. In the presence of the NADH dehydrogenase I (NDH-I) inhibitor 8-methyl-N- vanillyl-6-nonenamide (capsaicin) or in mutants lacking NDH-I, this ratio decreased to 1 for O2 and to 0 for DMSO. These data suggest that (i) the H+/e- stoichiometry for E. coli NDH-I is at least 1.5 and (ii) the DMSO reductase does not generate a proton motive force.
AB - Anaerobically grown Escherichia coli cells were shown to acidify the reaction medium in response to oxygen or dimethyl sulfoxide (DMSO) pulses, with the H+/e- stoichiometry being close to 2.5 and 1.5, respectively. In the presence of the NADH dehydrogenase I (NDH-I) inhibitor 8-methyl-N- vanillyl-6-nonenamide (capsaicin) or in mutants lacking NDH-I, this ratio decreased to 1 for O2 and to 0 for DMSO. These data suggest that (i) the H+/e- stoichiometry for E. coli NDH-I is at least 1.5 and (ii) the DMSO reductase does not generate a proton motive force.
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U2 - 10.1128/jb.178.21.6233-6237.1996
DO - 10.1128/jb.178.21.6233-6237.1996
M3 - Article
C2 - 8892824
AN - SCOPUS:0029910514
SN - 0021-9193
VL - 178
SP - 6233
EP - 6237
JO - Journal of bacteriology
JF - Journal of bacteriology
IS - 21
ER -