H+/e- stoichiometry for NADH dehydrogenase I and dimethyl sulfoxide reductase in anaerobically grown Escherichia coli cells

Alexander V. Bogachev, Rakhilya A. Murtazina, Vladimir P. Skulachev

Research output: Contribution to journalArticle

Abstract

Anaerobically grown Escherichia coli cells were shown to acidify the reaction medium in response to oxygen or dimethyl sulfoxide (DMSO) pulses, with the H+/e- stoichiometry being close to 2.5 and 1.5, respectively. In the presence of the NADH dehydrogenase I (NDH-I) inhibitor 8-methyl-N- vanillyl-6-nonenamide (capsaicin) or in mutants lacking NDH-I, this ratio decreased to 1 for O2 and to 0 for DMSO. These data suggest that (i) the H+/e- stoichiometry for E. coli NDH-I is at least 1.5 and (ii) the DMSO reductase does not generate a proton motive force.

Original languageEnglish (US)
Pages (from-to)6233-6237
Number of pages5
JournalJournal of bacteriology
Volume178
Issue number21
DOIs
StatePublished - Jan 1 1996

ASJC Scopus subject areas

  • Microbiology
  • Molecular Biology

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