HPV16 L1 capsid protein expressed from viable adenovirus recombinants elicits neutralizing antibody in mice

Michael Berg; Ratish Gambhira; Mark Siracusa; Egbert Hoiczyk; Richard Roden; Gary Ketner

doi:10.1016/j.vaccine.2006.06.080

HPV16 L1 capsid protein expressed from viable adenovirus recombinants elicits neutralizing antibody in mice

Michael Berg, Ratish Gambhira, Mark Siracusa, Egbert Hoiczyk, Richard Roden, Gary Ketner

Research output: Contribution to journal › Article › peer-review

9 Scopus citations

Abstract

Immunization against human papillomavirus (HPV) infection promises to reduce the worldwide burden of cervical cancer. To evaluate the potential of live recombinant adenoviruses for induction of HPV infection-blocking immunity, we prepared viable adenovirus recombinants that express the HPV16 L1 gene from the adenovirus major late transcriptional unit. Adenovirus-produced HPV16 L1 assembles into virus-like particles (VLPs) in infected cells in culture. Purified HPV16 VLPs are recognized by HPV16 neutralizing antibodies and induce high neutralizing titers when injected intraperitoneally into mice. Canine oral papillomavirus VLPs derived from previously described recombinants also induce strong antibody responses in mice. These data support our suggestion that viable adenovirus recombinants will be able to induce protective immunity to papillomavirus infection during replication in human vaccinees.

Original language	English (US)
Pages (from-to)	3501-3510
Number of pages	10
Journal	Vaccine
Volume	25
Issue number	17
DOIs	https://doi.org/10.1016/j.vaccine.2006.06.080
State	Published - Apr 30 2007

Keywords

HPV
Immunogenicity
Recombinant adenovirus vaccine

ASJC Scopus subject areas

Molecular Medicine
General Immunology and Microbiology
General Veterinary
Public Health, Environmental and Occupational Health
Infectious Diseases

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10.1016/j.vaccine.2006.06.080

Cite this

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title = "HPV16 L1 capsid protein expressed from viable adenovirus recombinants elicits neutralizing antibody in mice",

abstract = "Immunization against human papillomavirus (HPV) infection promises to reduce the worldwide burden of cervical cancer. To evaluate the potential of live recombinant adenoviruses for induction of HPV infection-blocking immunity, we prepared viable adenovirus recombinants that express the HPV16 L1 gene from the adenovirus major late transcriptional unit. Adenovirus-produced HPV16 L1 assembles into virus-like particles (VLPs) in infected cells in culture. Purified HPV16 VLPs are recognized by HPV16 neutralizing antibodies and induce high neutralizing titers when injected intraperitoneally into mice. Canine oral papillomavirus VLPs derived from previously described recombinants also induce strong antibody responses in mice. These data support our suggestion that viable adenovirus recombinants will be able to induce protective immunity to papillomavirus infection during replication in human vaccinees.",

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T1 - HPV16 L1 capsid protein expressed from viable adenovirus recombinants elicits neutralizing antibody in mice

AU - Berg, Michael

AU - Gambhira, Ratish

AU - Siracusa, Mark

AU - Hoiczyk, Egbert

AU - Roden, Richard

AU - Ketner, Gary

PY - 2007/4/30

Y1 - 2007/4/30

N2 - Immunization against human papillomavirus (HPV) infection promises to reduce the worldwide burden of cervical cancer. To evaluate the potential of live recombinant adenoviruses for induction of HPV infection-blocking immunity, we prepared viable adenovirus recombinants that express the HPV16 L1 gene from the adenovirus major late transcriptional unit. Adenovirus-produced HPV16 L1 assembles into virus-like particles (VLPs) in infected cells in culture. Purified HPV16 VLPs are recognized by HPV16 neutralizing antibodies and induce high neutralizing titers when injected intraperitoneally into mice. Canine oral papillomavirus VLPs derived from previously described recombinants also induce strong antibody responses in mice. These data support our suggestion that viable adenovirus recombinants will be able to induce protective immunity to papillomavirus infection during replication in human vaccinees.

AB - Immunization against human papillomavirus (HPV) infection promises to reduce the worldwide burden of cervical cancer. To evaluate the potential of live recombinant adenoviruses for induction of HPV infection-blocking immunity, we prepared viable adenovirus recombinants that express the HPV16 L1 gene from the adenovirus major late transcriptional unit. Adenovirus-produced HPV16 L1 assembles into virus-like particles (VLPs) in infected cells in culture. Purified HPV16 VLPs are recognized by HPV16 neutralizing antibodies and induce high neutralizing titers when injected intraperitoneally into mice. Canine oral papillomavirus VLPs derived from previously described recombinants also induce strong antibody responses in mice. These data support our suggestion that viable adenovirus recombinants will be able to induce protective immunity to papillomavirus infection during replication in human vaccinees.

KW - HPV

KW - Immunogenicity

KW - Recombinant adenovirus vaccine

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HPV16 L1 capsid protein expressed from viable adenovirus recombinants elicits neutralizing antibody in mice

Abstract

Keywords

ASJC Scopus subject areas

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