How biofilms evade host defenses

Emmanuel Roilides, Maria Simitsopoulou, Aspasia Katragkou, Thomas J. Walsh

Research output: Contribution to journalArticle

Abstract

The steps involved during the biofilm growth cycle include attachment to a substrate followed by more permanent adherence of the microorganisms, microcolony arrangement, and cell detachment required for the dissemination of single or clustered cells to other organ systems. Various methods have been developed for biofilm detection and quantitation. Biofilm-producing microorganisms can be detected in tissue culture plates, using silicone tubes and staining methods, and by visual assessment using scanning electron microscopy or confocal scanning laser microscopy. Quantitative measurement of biofilm growth is determined by using methods that include dry cell weight assays, colony-forming-unit counting, DNA quantification, or XTT 2,3-bis (2-methoxy-4-nitro-5-sulfophenyl)-5-[(phenylamino) carbonyl]-2H-tetrazolium hydroxide reduction assay. Upon infection, innate immune defense strategies are able to establish an immediate response through effector mechanisms mediated by immune cells, receptors, and several humoral factors. We present an overview of the life cycle of biofilms and their diversity, detection methods for biofilm development, and host immune responses to pathogens. We then focus on current concepts in bacterial and fungal biofilm immune evasion mechanisms. This appears to be of particular importance because the use of host immune responses may represent a novel therapeutic approach against biofilms.

Original languageEnglish (US)
Article numberMB-0012-2014
JournalMicrobiology spectrum
Volume3
Issue number3
DOIs
StatePublished - 2015
Externally publishedYes

Fingerprint

Biofilms
biofilm
immune response
microorganism
Colony-Forming Units Assay
assay
Immune Evasion
defence
Silicones
Growth
detection method
Life Cycle Stages
Confocal Microscopy
Electron Scanning Microscopy
hydroxide
microscopy
life cycle
pathogen
laser
scanning electron microscopy

ASJC Scopus subject areas

  • Immunology and Microbiology(all)
  • Infectious Diseases
  • Microbiology (medical)
  • Ecology
  • Cell Biology
  • Genetics
  • Physiology

Cite this

Roilides, E., Simitsopoulou, M., Katragkou, A., & Walsh, T. J. (2015). How biofilms evade host defenses. Microbiology spectrum, 3(3), [MB-0012-2014]. https://doi.org/10.1128/microbiolspec.MB-0012-2014

How biofilms evade host defenses. / Roilides, Emmanuel; Simitsopoulou, Maria; Katragkou, Aspasia; Walsh, Thomas J.

In: Microbiology spectrum, Vol. 3, No. 3, MB-0012-2014, 2015.

Research output: Contribution to journalArticle

Roilides, E, Simitsopoulou, M, Katragkou, A & Walsh, TJ 2015, 'How biofilms evade host defenses', Microbiology spectrum, vol. 3, no. 3, MB-0012-2014. https://doi.org/10.1128/microbiolspec.MB-0012-2014
Roilides E, Simitsopoulou M, Katragkou A, Walsh TJ. How biofilms evade host defenses. Microbiology spectrum. 2015;3(3). MB-0012-2014. https://doi.org/10.1128/microbiolspec.MB-0012-2014
Roilides, Emmanuel ; Simitsopoulou, Maria ; Katragkou, Aspasia ; Walsh, Thomas J. / How biofilms evade host defenses. In: Microbiology spectrum. 2015 ; Vol. 3, No. 3.
@article{21a02379366d4debad1d6a91284132b6,
title = "How biofilms evade host defenses",
abstract = "The steps involved during the biofilm growth cycle include attachment to a substrate followed by more permanent adherence of the microorganisms, microcolony arrangement, and cell detachment required for the dissemination of single or clustered cells to other organ systems. Various methods have been developed for biofilm detection and quantitation. Biofilm-producing microorganisms can be detected in tissue culture plates, using silicone tubes and staining methods, and by visual assessment using scanning electron microscopy or confocal scanning laser microscopy. Quantitative measurement of biofilm growth is determined by using methods that include dry cell weight assays, colony-forming-unit counting, DNA quantification, or XTT 2,3-bis (2-methoxy-4-nitro-5-sulfophenyl)-5-[(phenylamino) carbonyl]-2H-tetrazolium hydroxide reduction assay. Upon infection, innate immune defense strategies are able to establish an immediate response through effector mechanisms mediated by immune cells, receptors, and several humoral factors. We present an overview of the life cycle of biofilms and their diversity, detection methods for biofilm development, and host immune responses to pathogens. We then focus on current concepts in bacterial and fungal biofilm immune evasion mechanisms. This appears to be of particular importance because the use of host immune responses may represent a novel therapeutic approach against biofilms.",
author = "Emmanuel Roilides and Maria Simitsopoulou and Aspasia Katragkou and Walsh, {Thomas J.}",
year = "2015",
doi = "10.1128/microbiolspec.MB-0012-2014",
language = "English (US)",
volume = "3",
journal = "Microbiology spectrum",
issn = "2165-0497",
publisher = "American Society for Microbiology",
number = "3",

}

TY - JOUR

T1 - How biofilms evade host defenses

AU - Roilides, Emmanuel

AU - Simitsopoulou, Maria

AU - Katragkou, Aspasia

AU - Walsh, Thomas J.

PY - 2015

Y1 - 2015

N2 - The steps involved during the biofilm growth cycle include attachment to a substrate followed by more permanent adherence of the microorganisms, microcolony arrangement, and cell detachment required for the dissemination of single or clustered cells to other organ systems. Various methods have been developed for biofilm detection and quantitation. Biofilm-producing microorganisms can be detected in tissue culture plates, using silicone tubes and staining methods, and by visual assessment using scanning electron microscopy or confocal scanning laser microscopy. Quantitative measurement of biofilm growth is determined by using methods that include dry cell weight assays, colony-forming-unit counting, DNA quantification, or XTT 2,3-bis (2-methoxy-4-nitro-5-sulfophenyl)-5-[(phenylamino) carbonyl]-2H-tetrazolium hydroxide reduction assay. Upon infection, innate immune defense strategies are able to establish an immediate response through effector mechanisms mediated by immune cells, receptors, and several humoral factors. We present an overview of the life cycle of biofilms and their diversity, detection methods for biofilm development, and host immune responses to pathogens. We then focus on current concepts in bacterial and fungal biofilm immune evasion mechanisms. This appears to be of particular importance because the use of host immune responses may represent a novel therapeutic approach against biofilms.

AB - The steps involved during the biofilm growth cycle include attachment to a substrate followed by more permanent adherence of the microorganisms, microcolony arrangement, and cell detachment required for the dissemination of single or clustered cells to other organ systems. Various methods have been developed for biofilm detection and quantitation. Biofilm-producing microorganisms can be detected in tissue culture plates, using silicone tubes and staining methods, and by visual assessment using scanning electron microscopy or confocal scanning laser microscopy. Quantitative measurement of biofilm growth is determined by using methods that include dry cell weight assays, colony-forming-unit counting, DNA quantification, or XTT 2,3-bis (2-methoxy-4-nitro-5-sulfophenyl)-5-[(phenylamino) carbonyl]-2H-tetrazolium hydroxide reduction assay. Upon infection, innate immune defense strategies are able to establish an immediate response through effector mechanisms mediated by immune cells, receptors, and several humoral factors. We present an overview of the life cycle of biofilms and their diversity, detection methods for biofilm development, and host immune responses to pathogens. We then focus on current concepts in bacterial and fungal biofilm immune evasion mechanisms. This appears to be of particular importance because the use of host immune responses may represent a novel therapeutic approach against biofilms.

UR - http://www.scopus.com/inward/record.url?scp=84959045766&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=84959045766&partnerID=8YFLogxK

U2 - 10.1128/microbiolspec.MB-0012-2014

DO - 10.1128/microbiolspec.MB-0012-2014

M3 - Article

C2 - 26185085

AN - SCOPUS:84959045766

VL - 3

JO - Microbiology spectrum

JF - Microbiology spectrum

SN - 2165-0497

IS - 3

M1 - MB-0012-2014

ER -