Hormones increase mRNA of cyclic-nucleotide-gated cation channels in airway epithelia

W. Qiu, A. Laheri, Steve Leung, S. E. Guggino

Research output: Contribution to journalArticle

Abstract

Previous studies have shown that the mRNA of cyclic-nucleotide-gated nonselective cation (CNG) channels is expressed in rat airway epithelia and that these channels contribute to sodium-mediated short-circuit currents in cultured rat tracheal epithelia. Patch-clamp studies from human A549 cells indicate that these channels contribute to cGMP-stimulated L-cis-diltiazem- and dichlorobenzamil-inhibited whole-cell sodium currents. This study demonstrates that mRNA for primary and secondary subunits of CNG channels, hαCNG1 and hβCNG1 respectively, are expressed in several human airway cell lines, including normal and cystic fibrosis bronchial airway cells, in normal and cystic fibrosis tracheal airway cell lines and nasal polyp tissue from a cystic fibrosis patient. The mRNA of rαCNG1 in rat lung increased in response to increased circulating glucocorticoids and decreased in animals with lowered circulating glucocorticoids after aminoglutethimide treatment. Likewise the mRNA of hαCNG1 increased in the presence of glucocorticoids in cultured alveolar airway cells. The mRNA of αCNG1 in rat lung was also increased in response to a low-salt diet and lowered in animals fed a high-salt diet. Likewise the mRNA of αCNG1 was increased in response to increased aldosterone and decreased in animals given spironolactone. These results suggest that mRNA for αCNG1 increases in response to elevated glucocorticoids or mineralocorticoids. Because αCNG1 is a functional sodium entry channel in both rat and human airway epithelial cells, if channel protein is also elevated this channel could mediate an increase in sodium absorption across lung epithelia in response to circulating hormones.

Original languageEnglish (US)
Pages (from-to)69-77
Number of pages9
JournalPflugers Archiv European Journal of Physiology
Volume441
Issue number1
DOIs
StatePublished - 2000

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Cyclic Nucleotide-Gated Cation Channels
Epithelium
Hormones
Rats
Messenger RNA
Glucocorticoids
Sodium
Cystic Fibrosis
Animals
Nutrition
Lung
Salts
Cells
Aminoglutethimide
Alveolar Epithelial Cells
Sodium-Restricted Diet
Nasal Polyps
Cell Line
Mineralocorticoids
Spironolactone

Keywords

  • Lung sodium absorption
  • Nonselective cation channels
  • Sodium absorption
  • Sodium channels

ASJC Scopus subject areas

  • Physiology

Cite this

Hormones increase mRNA of cyclic-nucleotide-gated cation channels in airway epithelia. / Qiu, W.; Laheri, A.; Leung, Steve; Guggino, S. E.

In: Pflugers Archiv European Journal of Physiology, Vol. 441, No. 1, 2000, p. 69-77.

Research output: Contribution to journalArticle

Qiu, W. ; Laheri, A. ; Leung, Steve ; Guggino, S. E. / Hormones increase mRNA of cyclic-nucleotide-gated cation channels in airway epithelia. In: Pflugers Archiv European Journal of Physiology. 2000 ; Vol. 441, No. 1. pp. 69-77.
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