On of the characteristics of suckling rat Intestine Is the high plnocytlc activity leading to the absorption of Intact macromolecules. The process Is Influenced by hormones, nutritional status and age of the animals. To explore the role of these factors In the absorption of IgG, the binding of I(125)labelled IgG to Its receptor(PoRn) In brush borders Isolated from 8-30 day old rats was studled. The binding of IgG to the membranes gradually reduced from day 8 to negligible levels in adult animals. In suckling pups, IgG binding was saturable and Scatchard analysis revealed that protein with an affinity constant of 1.53x10°"- , with binding sltesfnJseOnmoles/mg protein. Administration of cortlsone(5mg/100g b.wt) or thyroxlne ( ?5ue;/100f; b.wt) dally for three days to 12 day old pups narkedly reduced the IgG blnding(p <: 0.01). Deslalylatlon of membranes using neuramlnldase also reduced(p <0.01) IgG binding, compared to controls. These observations suggest the role of surface slallc acid in the adhesion of IgG to its receptor in mlcrovillus surface of suckling rat small intestine.
|Original language||English (US)|
|State||Published - Dec 1 1997|
ASJC Scopus subject areas
- Molecular Biology