Hormonal, drug, and dietary factors affecting peptidyl glycine α-amidating monooxygenase activity in various tissues of the adult male rat

R. E. Mains, A. C. Myers, B. A. Eipper

Research output: Contribution to journalArticle

Abstract

The factors controlling levels of peptidyl glycine α-amidating monooxygenase (PAM) activity in its major tissue sources in the adult male rat were investigated by carrying out a variety of endocrine, pharmacological, and dietary manipulations. Levels of PAM activity and αMSH immunoactivity in the neurointermediate lobe of the pituitary gland rose and fell in parallel in rats treated with the dopamine antagonist haloperidol or the dopamine agonist bromocriptine, respectively. PAM activity in the anterior pituitary lobe was increased after adrenalectomy or castration and decreased after thyroidectomy or treatment with haloperidol. PAM activity in the submandibular gland was increased after treatment with the α-adrenergic antagonist phenoxybenzamine and decreased after treatment with the α-adrenergic agonist phenylephrine. Serum levels of PAM activity were unaltered after hypophysectomy, adrenalectomy, sialectomy, or castration, but rose after thyroidectomy and declined after treatment with the ganglionic blocker chlorisondamine or phenoxybenzamine. Chronic dietary copper deficiency in rats resulted in increased PAM activity in homogenates of anterior pituitary lobe and submandibular gland assayed under optimized conditions; chronic dietary ascorbate deficiency in guinea pigs did not produce consistent changes in PAM activity in the tissues examined.

Original languageEnglish (US)
Pages (from-to)2505-2515
Number of pages11
JournalEndocrinology
Volume116
Issue number6
StatePublished - 1985

Fingerprint

Mixed Function Oxygenases
Glycine
Pharmaceutical Preparations
Phenoxybenzamine
Anterior Pituitary Gland
Submandibular Gland
Castration
Adrenalectomy
Thyroidectomy
Haloperidol
Chlorisondamine
Ganglionic Blockers
Melanocyte-Stimulating Hormones
Hypophysectomy
Adrenergic Agonists
Adrenergic Antagonists
Bromocriptine
Dopamine Antagonists
Dopamine Agonists
Phenylephrine

ASJC Scopus subject areas

  • Endocrinology
  • Endocrinology, Diabetes and Metabolism

Cite this

Hormonal, drug, and dietary factors affecting peptidyl glycine α-amidating monooxygenase activity in various tissues of the adult male rat. / Mains, R. E.; Myers, A. C.; Eipper, B. A.

In: Endocrinology, Vol. 116, No. 6, 1985, p. 2505-2515.

Research output: Contribution to journalArticle

@article{afb86ccb631d4ca0a8afc3c263ae3605,
title = "Hormonal, drug, and dietary factors affecting peptidyl glycine α-amidating monooxygenase activity in various tissues of the adult male rat",
abstract = "The factors controlling levels of peptidyl glycine α-amidating monooxygenase (PAM) activity in its major tissue sources in the adult male rat were investigated by carrying out a variety of endocrine, pharmacological, and dietary manipulations. Levels of PAM activity and αMSH immunoactivity in the neurointermediate lobe of the pituitary gland rose and fell in parallel in rats treated with the dopamine antagonist haloperidol or the dopamine agonist bromocriptine, respectively. PAM activity in the anterior pituitary lobe was increased after adrenalectomy or castration and decreased after thyroidectomy or treatment with haloperidol. PAM activity in the submandibular gland was increased after treatment with the α-adrenergic antagonist phenoxybenzamine and decreased after treatment with the α-adrenergic agonist phenylephrine. Serum levels of PAM activity were unaltered after hypophysectomy, adrenalectomy, sialectomy, or castration, but rose after thyroidectomy and declined after treatment with the ganglionic blocker chlorisondamine or phenoxybenzamine. Chronic dietary copper deficiency in rats resulted in increased PAM activity in homogenates of anterior pituitary lobe and submandibular gland assayed under optimized conditions; chronic dietary ascorbate deficiency in guinea pigs did not produce consistent changes in PAM activity in the tissues examined.",
author = "Mains, {R. E.} and Myers, {A. C.} and Eipper, {B. A.}",
year = "1985",
language = "English (US)",
volume = "116",
pages = "2505--2515",
journal = "Endocrinology",
issn = "0013-7227",
publisher = "The Endocrine Society",
number = "6",

}

TY - JOUR

T1 - Hormonal, drug, and dietary factors affecting peptidyl glycine α-amidating monooxygenase activity in various tissues of the adult male rat

AU - Mains, R. E.

AU - Myers, A. C.

AU - Eipper, B. A.

PY - 1985

Y1 - 1985

N2 - The factors controlling levels of peptidyl glycine α-amidating monooxygenase (PAM) activity in its major tissue sources in the adult male rat were investigated by carrying out a variety of endocrine, pharmacological, and dietary manipulations. Levels of PAM activity and αMSH immunoactivity in the neurointermediate lobe of the pituitary gland rose and fell in parallel in rats treated with the dopamine antagonist haloperidol or the dopamine agonist bromocriptine, respectively. PAM activity in the anterior pituitary lobe was increased after adrenalectomy or castration and decreased after thyroidectomy or treatment with haloperidol. PAM activity in the submandibular gland was increased after treatment with the α-adrenergic antagonist phenoxybenzamine and decreased after treatment with the α-adrenergic agonist phenylephrine. Serum levels of PAM activity were unaltered after hypophysectomy, adrenalectomy, sialectomy, or castration, but rose after thyroidectomy and declined after treatment with the ganglionic blocker chlorisondamine or phenoxybenzamine. Chronic dietary copper deficiency in rats resulted in increased PAM activity in homogenates of anterior pituitary lobe and submandibular gland assayed under optimized conditions; chronic dietary ascorbate deficiency in guinea pigs did not produce consistent changes in PAM activity in the tissues examined.

AB - The factors controlling levels of peptidyl glycine α-amidating monooxygenase (PAM) activity in its major tissue sources in the adult male rat were investigated by carrying out a variety of endocrine, pharmacological, and dietary manipulations. Levels of PAM activity and αMSH immunoactivity in the neurointermediate lobe of the pituitary gland rose and fell in parallel in rats treated with the dopamine antagonist haloperidol or the dopamine agonist bromocriptine, respectively. PAM activity in the anterior pituitary lobe was increased after adrenalectomy or castration and decreased after thyroidectomy or treatment with haloperidol. PAM activity in the submandibular gland was increased after treatment with the α-adrenergic antagonist phenoxybenzamine and decreased after treatment with the α-adrenergic agonist phenylephrine. Serum levels of PAM activity were unaltered after hypophysectomy, adrenalectomy, sialectomy, or castration, but rose after thyroidectomy and declined after treatment with the ganglionic blocker chlorisondamine or phenoxybenzamine. Chronic dietary copper deficiency in rats resulted in increased PAM activity in homogenates of anterior pituitary lobe and submandibular gland assayed under optimized conditions; chronic dietary ascorbate deficiency in guinea pigs did not produce consistent changes in PAM activity in the tissues examined.

UR - http://www.scopus.com/inward/record.url?scp=0021802903&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0021802903&partnerID=8YFLogxK

M3 - Article

C2 - 3996325

AN - SCOPUS:0021802903

VL - 116

SP - 2505

EP - 2515

JO - Endocrinology

JF - Endocrinology

SN - 0013-7227

IS - 6

ER -