Homozygous deletion of MKK4 in ovarian serous carcinoma

Kentaro Nakayama, Naomi Nakayama, Ben Davidson, Hidetaka Katabuchi, Robert J. Kurman, Victor E. Velculescu, Ie Ming Shih, Tian Li Wang

Research output: Contribution to journalArticlepeer-review

Abstract

Analysis of deleted chromosomal regions in tumors has historically led to the identification of tumor suppressor genes. In this study, we used digital karyotyping, a genome-wide, high-resolution technology, to search for chromosomal deletions in ovarian serous carcinoma, the most lethal gynecological malignancy in women. Five purified ovarian serous carcinomas were analyzed by digital karyotyping and small interstitial deletions at chromosome 17p were identified in two tumor samples. Aligning these two deletions identified an overlapping region that spanned 2.4 Mb which harbored a candidate tumor suppressor gene, mitogen-activated protein kinase kinase-4 (MKK4). Dual-color FISH analysis confirmed homozygous deletion of the MKK4 locus in both samples and RT-PCR demonstrated that both carcinomas lacked MKK4 transcript expression. Loss of heterozygosity of 17p occurred in 24 (86%) of 28 high-grade serous carcinomas including both cases with homozygous MKK4 deletion. Additionally, downregulation of MKK4 expression was found in 96 (75%) of 128 ovarian serous carcinomas as compared to benign ovarian tissues. These findings suggest that homozygous deletion or reduced expression of MKK4 may contribute to the development of ovarian serous carcinoma.

Original languageEnglish (US)
Pages (from-to)630-634
Number of pages5
JournalCancer Biology and Therapy
Volume5
Issue number6
DOIs
StatePublished - 2006

Keywords

  • Digital karyotyping
  • Homozygous deletion
  • Loss of heterozygosity
  • Ovarian cancer
  • Tumor suppressor

ASJC Scopus subject areas

  • Molecular Medicine
  • Oncology
  • Pharmacology
  • Cancer Research

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