Homozygous deletion map at 18q21.1 in pancreatic cancer

Stephan A. Hahn, A. T.M.Shamsul Hoque, Christopher A. Moskaluk, Luis T. Da Costa, Mieke Schutte, Ester Rozenblum, Albert B. Seymour, Craig L. Weinstein, Charles J. Yeo, Ralph H. Hruban, Scott E. Kern

Research output: Contribution to journalArticle

Abstract

Absolute genetic differences between neoplastic and nonneoplastic cells can be discerned at sites of homozygous deletions. These deletions are of critical interest because they might be useful in the identification of defective biochemical pathways in neoplastic cells, and subsequently for the development of new treatment strategies in human cancer. We identified an area at 18q21.1 involved by homozygous deletions in 30% of pancreatic carcinomas. To characterize the homozygous deletions, we constructed a detailed physical map of nearly 2 Mb, containing yeast artificial chromosomes, P1-derived artificial chromosomes, cosmids and 24 sequence- tagged sites. The homozygously deleted area contained a new candidate tumor- suppressor gene (DPC4). To date, 23 (64%) of 35 pancreatic carcinomas carry at least one homozygous deletion at a published locus. The study of the total gene content of these loci, facilitated by the sequence-tagged site markers and maps of these regions, should help to reveal the absolute biochemical differences between neoplastic and nonneoplastic cells for a common human tumor.

Original languageEnglish (US)
Pages (from-to)490-494
Number of pages5
JournalCancer Research
Volume56
Issue number3
StatePublished - Feb 1 1996

ASJC Scopus subject areas

  • Oncology
  • Cancer Research

Fingerprint Dive into the research topics of 'Homozygous deletion map at 18q21.1 in pancreatic cancer'. Together they form a unique fingerprint.

  • Cite this

    Hahn, S. A., Hoque, A. T. M. S., Moskaluk, C. A., Da Costa, L. T., Schutte, M., Rozenblum, E., Seymour, A. B., Weinstein, C. L., Yeo, C. J., Hruban, R. H., & Kern, S. E. (1996). Homozygous deletion map at 18q21.1 in pancreatic cancer. Cancer Research, 56(3), 490-494.