Abstract
The selection of T cell clones with mutations in the hypoxanthine guanine phosphoribosyltransferase (hprt) gene has been used to isolate T cells reactive to myelin basic protein (MBP) in patients with multiple sclerosis (MS). These T cell clones are activated in vivo, and are not found in healthy individuals. The third complementarity determining regions (CDR3) of the T cell receptor (TCR) α and β chains are the putative contact sites for peptide fragments of MBP bound in the groove of the HLA molecule. The TCR V gene usage and CDR3s of these MBP-reactive hprt- T cell clones are homologous to TCRs from other T cells relevant to MS, including T cells causing experimental allergic encephalomyelitis (EAE) and T cells found in brain lesions and in the cerebrospinal fluid (CSF) of MS patients. In vivo activated MBP-reactive T cells in MS patients may be critical in the pathogenesis of MS.
Original language | English (US) |
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Pages (from-to) | 105-109 |
Number of pages | 5 |
Journal | Journal of Clinical Investigation |
Volume | 94 |
Issue number | 1 |
State | Published - Jul 1994 |
Externally published | Yes |
Keywords
- hypoxanthine phosphoribosyltransferase
- in vivo
- mutation
- myelin basic protein
- T lymphocytes
ASJC Scopus subject areas
- General Medicine