Homologies between T cell receptor junctional sequences unique to multiple sclerosis and T cells mediating experimental allergic encephalomyelitis

Mark Allegretta, Richard J. Albertini, Mark D. Howell, Lawrence R. Smith, Roland Martin, Henry F. McFarland, Subramamiam Sriram, Steven Brostoff, Lawrence Steinman

Research output: Contribution to journalArticle

Abstract

The selection of T cell clones with mutations in the hypoxanthine guanine phosphoribosyltransferase (hprt) gene has been used to isolate T cells reactive to myelin basic protein (MBP) in patients with multiple sclerosis (MS). These T cell clones are activated in vivo, and are not found in healthy individuals. The third complementarity determining regions (CDR3) of the T cell receptor (TCR) α and β chains are the putative contact sites for peptide fragments of MBP bound in the groove of the HLA molecule. The TCR V gene usage and CDR3s of these MBP-reactive hprt- T cell clones are homologous to TCRs from other T cells relevant to MS, including T cells causing experimental allergic encephalomyelitis (EAE) and T cells found in brain lesions and in the cerebrospinal fluid (CSF) of MS patients. In vivo activated MBP-reactive T cells in MS patients may be critical in the pathogenesis of MS.

Original languageEnglish (US)
Pages (from-to)105-109
Number of pages5
JournalJournal of Clinical Investigation
Volume94
Issue number1
StatePublished - Jul 1994
Externally publishedYes

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Autoimmune Experimental Encephalomyelitis
T-Cell Antigen Receptor
Multiple Sclerosis
T-Lymphocytes
Myelin Basic Protein
Hypoxanthine Phosphoribosyltransferase
Clone Cells
Complementarity Determining Regions
T-Cell Receptor Genes
Peptide Fragments
Cerebrospinal Fluid
Mutation
Brain

Keywords

  • hypoxanthine phosphoribosyltransferase
  • in vivo
  • mutation
  • myelin basic protein
  • T lymphocytes

ASJC Scopus subject areas

  • Medicine(all)

Cite this

Allegretta, M., Albertini, R. J., Howell, M. D., Smith, L. R., Martin, R., McFarland, H. F., ... Steinman, L. (1994). Homologies between T cell receptor junctional sequences unique to multiple sclerosis and T cells mediating experimental allergic encephalomyelitis. Journal of Clinical Investigation, 94(1), 105-109.

Homologies between T cell receptor junctional sequences unique to multiple sclerosis and T cells mediating experimental allergic encephalomyelitis. / Allegretta, Mark; Albertini, Richard J.; Howell, Mark D.; Smith, Lawrence R.; Martin, Roland; McFarland, Henry F.; Sriram, Subramamiam; Brostoff, Steven; Steinman, Lawrence.

In: Journal of Clinical Investigation, Vol. 94, No. 1, 07.1994, p. 105-109.

Research output: Contribution to journalArticle

Allegretta, M, Albertini, RJ, Howell, MD, Smith, LR, Martin, R, McFarland, HF, Sriram, S, Brostoff, S & Steinman, L 1994, 'Homologies between T cell receptor junctional sequences unique to multiple sclerosis and T cells mediating experimental allergic encephalomyelitis', Journal of Clinical Investigation, vol. 94, no. 1, pp. 105-109.
Allegretta, Mark ; Albertini, Richard J. ; Howell, Mark D. ; Smith, Lawrence R. ; Martin, Roland ; McFarland, Henry F. ; Sriram, Subramamiam ; Brostoff, Steven ; Steinman, Lawrence. / Homologies between T cell receptor junctional sequences unique to multiple sclerosis and T cells mediating experimental allergic encephalomyelitis. In: Journal of Clinical Investigation. 1994 ; Vol. 94, No. 1. pp. 105-109.
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