Homing of long-term and short-term engrafting cells in vivo

Sophie Lanzkron, Michael I. Collector, Saul J. Sharkis

Research output: Contribution to journalArticle

Abstract

Long-term repopulating hematopoietic stem cells can be separated from cells which provided radioprotection (short-term repopulating cells) on the basis of size. This might be a result of the quiescent nature of long-term repopulating cells. To define the activity of these populations we utilized a dye, PKH26, which incorporates into the membrane of cells and is equally distributed to daughter cells when they divide. We were able to retrieve PKH26+-labeled cells posttransplant in the hematopoietic tissues of the recipients. We could also assess their cell cycle status and their ability, short-and long-term, to reconstitute secondary lethally irradiated hosts in limiting dilution. The results suggest that long-term repopulating cells remain quiescent in the bone marrow shortly after engraftment, whereas cells which radioprotect are more rapidly dividing. We could not detect labeled cells in the peripheral blood posttransplant, and even though cells homed to both the spleen and bone marrow the cells in the bone marrow were significantly more competent at reconstituting lethally irradiated secondary hosts.

Original languageEnglish (US)
Pages (from-to)48-56
Number of pages9
JournalAnnals of the New York Academy of Sciences
Volume872
DOIs
StatePublished - 1999

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Bone
Stem cells
Dilution
Blood
Coloring Agents
Cells
Tissue
Membranes
Bone Marrow
Hematopoietic Stem Cells
Bone Marrow Cells
PKH 26
Cell Cycle
Spleen
Cell Membrane
Population

ASJC Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology(all)

Cite this

Homing of long-term and short-term engrafting cells in vivo. / Lanzkron, Sophie; Collector, Michael I.; Sharkis, Saul J.

In: Annals of the New York Academy of Sciences, Vol. 872, 1999, p. 48-56.

Research output: Contribution to journalArticle

Lanzkron, Sophie ; Collector, Michael I. ; Sharkis, Saul J. / Homing of long-term and short-term engrafting cells in vivo. In: Annals of the New York Academy of Sciences. 1999 ; Vol. 872. pp. 48-56.
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