Homer proteins regulate coupling of group I metabotropic glutamate receptors to N-type calcium and M-type potassium channels

P. J. Kammermeier, Bo Xiao, Cheng Tu Jian Cheng Tu, Paul F Worley, S. R. Ikeda

Research output: Contribution to journalArticle

Abstract

Group I metabotropic glutamate receptors (mGluR1 and 5) couple to intracellular calcium pools by a family of proteins, termed Homer, that cross-link the receptor to inositol trisphosphate receptors, mGluRs also couple to membrane on channels via G-proteins. The role of Homer proteins in channel modulation was investigated by expressing mGluRs and various forms of Homer in rat superior cervical ganglion (SCG) sympathetic neurons by intranuclear cDNA injection. Expression of cross-linking-capable forms of Homer (Homer 1b, 1c, 2, and 3, termed long forms) occluded group I mGluR-mediated N-type calcium and M-type potassium current modulation. This effect was specific for group I mGluRs, mGluR2 (group II)-mediated inhibition of N-channels was unaltered. Long forms of Homer decreased modulation of N- and M-type currents but did not selectively block distinct G-protein pathways. Short forms of Homer, which cannot self-multimerize (Homer 1a and a Homer 2 C-terminal deletion), did not alter mGluR-ion channel coupling. When co-expressed with long forms of Homer, short forms restored the mGluR1a-mediated calcium current modulation in an apparent dose-dependent manner. Homer 2b induced cell surface clusters of mGluR5 in SCG neurons. Conversely, a uniform distribution was observed when mGluR5 was expressed alone or with Homer short forms. These studies indicate that long and short forms of Homer compete for binding to mGluRs and regulate their coupling to ion channels. In vivo, the immediate early Homer 1a is anticipated to enhance ion channel modulation and to disrupt coupling to releasable intracellular calcium pools. Thus, Homer may regulate the magnitude and predominate signaling output of group I mGluRs.

Original languageEnglish (US)
Pages (from-to)7238-7245
Number of pages8
JournalJournal of Neuroscience
Volume20
Issue number19
Publication statusPublished - Oct 1 2000
Externally publishedYes

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Keywords

  • Calcium current
  • G-protein
  • Homer
  • Ion channel modulation
  • M-current
  • mGluR
  • Neuron

ASJC Scopus subject areas

  • Neuroscience(all)

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