HMG-I/Y in human breast cancer cell lines

Christine E. Dolde, Mita Mukherjee, Chung Cho, Linda M Smith Resar

Research output: Contribution to journalArticle

Abstract

The HMG-I/Y gene encodes the HMG-I and -Y architectural, chromatin binding proteins originally identified based on their association with chromosomal DNA. HMG-I/Y proteins bind to AT-rich regions in chromosomal DNA and alter gene expression. Increased HMG-I/Y protein expression also correlates with neoplastic transformation. Previous work from our laboratory has shown that HMG-I/Y is a direct c-Myc target gene involved in neoplastic transformation in Burkitt's lymphoma. We also observed that HMG-I/Y proteins have several oncogenic properties. In this report, we show that HMG-I/Y proteins are increased in several human breast cancer cell lines compared to a human breast cell line derived from normal breast cells. Decreasing HMG-I/Y proteins using an antisense ribozyme approach inhibits transformation in human breast cancer cells, suggesting that HMG-I/Y is important for the transformed phenotype observed in these cells. In addition, increased expression of the HMG-I isoform in normal human breast cells leads to transformation. These results suggest that HMG-I/Y is an oncogene important in the pathogenesis of human breast cancer. Although additional studies with animal models are needed, the antisense experiments, which result in blocking transformation suggest that this approach may have therapeutic potential in patients with breast cancer characterized by increased HMG-I/Y expression.

Original languageEnglish (US)
Pages (from-to)181-191
Number of pages11
JournalBreast Cancer Research and Treatment
Volume71
Issue number3
DOIs
StatePublished - 2002

Fingerprint

HMGA1a Protein
Breast Neoplasms
Cell Line
Breast
AT Rich Sequence
Catalytic RNA
myc Genes
Burkitt Lymphoma
DNA
Oncogenes
Chromatin
Carrier Proteins
Protein Isoforms
Animal Models
Phenotype
Gene Expression
Genes

Keywords

  • Antisense
  • Breast cancer
  • HMG-I/Y
  • Oncogene
  • Transformation

ASJC Scopus subject areas

  • Oncology
  • Cancer Research

Cite this

HMG-I/Y in human breast cancer cell lines. / Dolde, Christine E.; Mukherjee, Mita; Cho, Chung; Smith Resar, Linda M.

In: Breast Cancer Research and Treatment, Vol. 71, No. 3, 2002, p. 181-191.

Research output: Contribution to journalArticle

Dolde, Christine E. ; Mukherjee, Mita ; Cho, Chung ; Smith Resar, Linda M. / HMG-I/Y in human breast cancer cell lines. In: Breast Cancer Research and Treatment. 2002 ; Vol. 71, No. 3. pp. 181-191.
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