HMG Box-Activating Factors 1 and 2, Two HMG Box Transcription Factors That Bind the Human Ig Heavy Chain Enhancer

Sean Stevens, Peter Ordentlich, Ranjan Sen, Tom Kadesch

Research output: Contribution to journalArticle

Abstract

We present the isolation of two cDNAs that encode distinct, yet related, proteins that bind the HE2 region of the human Ig heavy chain (IgH) enhancer. Designated HMG box-activating factors (HAF) 1 and 2, the two proteins are new members of the HMG box family of DNA binding proteins. Both are potent transcription activators when expressed 1) as GAL4 fusions targeted to promoters containing GAL4 operators, or 2) as intact proteins targeted to minimal promoters containing binding sites derived from the IgH enhancer. HAF-1 and HAF-2 mRNAs are apparently expressed in both B cells and non-B cells. However, activity generated by the isolated HE2 region in B cells is dependent on both an intact HAF-1/HAF-2 binding site and at least one additional site that has been reported previously to bind a B cell-restricted protein. Our results suggest a collaborative role for either or both HAF-1 and HAF-2 in establishing the B cell activity of the human IgH enhancer.

Original languageEnglish (US)
Pages (from-to)3491-3498
Number of pages8
JournalJournal of Immunology
Volume157
Issue number8
StatePublished - Oct 15 1996
Externally publishedYes

Fingerprint

HMGB2 Protein
Immunoglobulin Heavy Chains
B-Lymphocytes
Transcription Factors
Proteins
Binding Sites
DNA-Binding Proteins
Human Activities
Complementary DNA
Messenger RNA

ASJC Scopus subject areas

  • Immunology

Cite this

HMG Box-Activating Factors 1 and 2, Two HMG Box Transcription Factors That Bind the Human Ig Heavy Chain Enhancer. / Stevens, Sean; Ordentlich, Peter; Sen, Ranjan; Kadesch, Tom.

In: Journal of Immunology, Vol. 157, No. 8, 15.10.1996, p. 3491-3498.

Research output: Contribution to journalArticle

Stevens, S, Ordentlich, P, Sen, R & Kadesch, T 1996, 'HMG Box-Activating Factors 1 and 2, Two HMG Box Transcription Factors That Bind the Human Ig Heavy Chain Enhancer', Journal of Immunology, vol. 157, no. 8, pp. 3491-3498.
Stevens, Sean ; Ordentlich, Peter ; Sen, Ranjan ; Kadesch, Tom. / HMG Box-Activating Factors 1 and 2, Two HMG Box Transcription Factors That Bind the Human Ig Heavy Chain Enhancer. In: Journal of Immunology. 1996 ; Vol. 157, No. 8. pp. 3491-3498.
@article{49997b7344b647919a3dea6b748932b3,
title = "HMG Box-Activating Factors 1 and 2, Two HMG Box Transcription Factors That Bind the Human Ig Heavy Chain Enhancer",
abstract = "We present the isolation of two cDNAs that encode distinct, yet related, proteins that bind the HE2 region of the human Ig heavy chain (IgH) enhancer. Designated HMG box-activating factors (HAF) 1 and 2, the two proteins are new members of the HMG box family of DNA binding proteins. Both are potent transcription activators when expressed 1) as GAL4 fusions targeted to promoters containing GAL4 operators, or 2) as intact proteins targeted to minimal promoters containing binding sites derived from the IgH enhancer. HAF-1 and HAF-2 mRNAs are apparently expressed in both B cells and non-B cells. However, activity generated by the isolated HE2 region in B cells is dependent on both an intact HAF-1/HAF-2 binding site and at least one additional site that has been reported previously to bind a B cell-restricted protein. Our results suggest a collaborative role for either or both HAF-1 and HAF-2 in establishing the B cell activity of the human IgH enhancer.",
author = "Sean Stevens and Peter Ordentlich and Ranjan Sen and Tom Kadesch",
year = "1996",
month = "10",
day = "15",
language = "English (US)",
volume = "157",
pages = "3491--3498",
journal = "Journal of Immunology",
issn = "0022-1767",
publisher = "American Association of Immunologists",
number = "8",

}

TY - JOUR

T1 - HMG Box-Activating Factors 1 and 2, Two HMG Box Transcription Factors That Bind the Human Ig Heavy Chain Enhancer

AU - Stevens, Sean

AU - Ordentlich, Peter

AU - Sen, Ranjan

AU - Kadesch, Tom

PY - 1996/10/15

Y1 - 1996/10/15

N2 - We present the isolation of two cDNAs that encode distinct, yet related, proteins that bind the HE2 region of the human Ig heavy chain (IgH) enhancer. Designated HMG box-activating factors (HAF) 1 and 2, the two proteins are new members of the HMG box family of DNA binding proteins. Both are potent transcription activators when expressed 1) as GAL4 fusions targeted to promoters containing GAL4 operators, or 2) as intact proteins targeted to minimal promoters containing binding sites derived from the IgH enhancer. HAF-1 and HAF-2 mRNAs are apparently expressed in both B cells and non-B cells. However, activity generated by the isolated HE2 region in B cells is dependent on both an intact HAF-1/HAF-2 binding site and at least one additional site that has been reported previously to bind a B cell-restricted protein. Our results suggest a collaborative role for either or both HAF-1 and HAF-2 in establishing the B cell activity of the human IgH enhancer.

AB - We present the isolation of two cDNAs that encode distinct, yet related, proteins that bind the HE2 region of the human Ig heavy chain (IgH) enhancer. Designated HMG box-activating factors (HAF) 1 and 2, the two proteins are new members of the HMG box family of DNA binding proteins. Both are potent transcription activators when expressed 1) as GAL4 fusions targeted to promoters containing GAL4 operators, or 2) as intact proteins targeted to minimal promoters containing binding sites derived from the IgH enhancer. HAF-1 and HAF-2 mRNAs are apparently expressed in both B cells and non-B cells. However, activity generated by the isolated HE2 region in B cells is dependent on both an intact HAF-1/HAF-2 binding site and at least one additional site that has been reported previously to bind a B cell-restricted protein. Our results suggest a collaborative role for either or both HAF-1 and HAF-2 in establishing the B cell activity of the human IgH enhancer.

UR - http://www.scopus.com/inward/record.url?scp=0030587852&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0030587852&partnerID=8YFLogxK

M3 - Article

C2 - 8871648

AN - SCOPUS:0030587852

VL - 157

SP - 3491

EP - 3498

JO - Journal of Immunology

JF - Journal of Immunology

SN - 0022-1767

IS - 8

ER -