HLA homozygous stem cell lines derived from human parthenogenetic blastocysts

E. S. Revazova, N. A. Turovets, O. D. Kochetkova, L. S. Agapova, J. L. Sebastian, M. V. Pryzhkova, V. Iu Smolnikova, L. N. Kuzmichev, J. D. Janus

Research output: Contribution to journalArticlepeer-review

115 Scopus citations

Abstract

Individual HLA homozygous parthenogenetic human stem cell (hpSC-Hhom) lines have the potential for cell-based therapy in a significant number of individuals, provided the HLA haplotype is prevalent. We report the successful derivation of four stable hpSC-Hhom lines from both HLA homozygous and HLA heterozygous donors. Of these, the hpSC-Hhom-4 line carries the HLA haplotype found most commonly within the U.S. population, and is shared by different racial groups. These hpSC-Hhom lines demonstrate typical human embryonic stem cell morphology, expressing appropriate stem cell markers and possessing high levels of alkaline phosphatase and telomerase activity. Additionally, injection of these cell lines into immunodeficient animals leads to teratoma formation. G-banded karyotyping demonstrates a normal 46,XX karyotype in lines hpSC-Hhom-1 and hpSC-Hhom-4, and chromosomal anomalies in lines hpSC-Hhom-2 and hpSC-Hhom-3, both derived from the same donor. HLA genotyping of all four hpSC-Hhom lines demonstrates that they are HLA homozygous. Furthermore, in the case of HLA heterozygous donors, the hpSC-Hhom lines inherit the haplotype from only one of the donor's parents. Single-nucleotide polymorphism (SNP) data analysis suggests that hpSC-Hhom lines derived from HLA heterozygous oocyte donors are homozygous throughout the genome as assessed by SNP analysis. The protocol used for deriving these HLA homozygous stem cell lines minimizes the use of animal-derived components, which makes them more appealing for potential clinical application.

Original languageEnglish (US)
Pages (from-to)11-24
Number of pages14
JournalCloning and Stem Cells
Volume10
Issue number1
DOIs
StatePublished - Mar 1 2008
Externally publishedYes

ASJC Scopus subject areas

  • Biotechnology
  • Developmental Biology

Fingerprint

Dive into the research topics of 'HLA homozygous stem cell lines derived from human parthenogenetic blastocysts'. Together they form a unique fingerprint.

Cite this