HLA-G upregulation in pre-malignant and malignant lesions of the gastrointestinal tract

Donna E. Hansel, Ayman Rahman, Robb E. Wilentz, Ie Ming Shih, Michael T. McMaster, Charles J. Yeo, Anriban Maitra

Research output: Contribution to journalArticle

Abstract

HLA-G belongs to the nonclassical MHC class Ib group of molecules and has been implicated in mediating immune-responsiveness in various cancerous and non-cancerous cell types. We have examined HLA-G expression in a number of human gastrointestinal malignancies, including pancreatic ductal adenocarcinoma, ampullary cancer, biliary cancer, and colorectal cancer by immunolabeling analysis. We used indices of 75% (diffuse) to subclassify lesions based on percentage of positive cell labeling. Across all cancer subtypes, 52-79% of lesions demonstrated expression of HLA-G, with up to 33% of lesions demonstrating diffuse (>75%) expression. In addition, we utilized the neoplastic progression model of colorectal cancer to evaluate HLA-G protein expression in normal colon, tubulovillous adenomas, invasive cancer, and liver metastases arising from colorectal cancer. Focal HLA-G expression was detected in regions of normal colon adjacent to sites of adenomatous and cancerous lesions, as well as in all stages of cancer progression. Overall, the percentage of diffusely (>75%) labeled lesions appeared increased in preneoplastic and neoplastic conditions, as compared to normal colon. Specifically, tubulovillous adnenomas demonstrated pronounced diffuse labeling in 58% of lesions examined. No correlation with HLA-G expression and CD4+ or CD8+ T cells was identified. We propose that HLA-G expression is upregulated in a large percentage of gastrointestinal lesions and may serve to mediate immune-responsiveness in certain instances.

Original languageEnglish (US)
Pages (from-to)15-23
Number of pages9
JournalInternational Journal of Gastrointestinal Cancer
Volume35
Issue number1
StatePublished - 2005

Fingerprint

HLA-G Antigens
Gastrointestinal Tract
Up-Regulation
Colorectal Neoplasms
Colon
Neoplasms
Precancerous Conditions
Biliary Tract Neoplasms
Liver Neoplasms
GTP-Binding Proteins
Adenoma
Adenocarcinoma
Neoplasm Metastasis
T-Lymphocytes

Keywords

  • Ampullary cancer
  • Biliary cancer
  • Colon
  • Colorectal cancer
  • Gallbladder
  • Immune
  • Pancreas
  • Pancreatic cancer
  • Tolerance

ASJC Scopus subject areas

  • Gastroenterology
  • Endocrinology
  • Oncology

Cite this

Hansel, D. E., Rahman, A., Wilentz, R. E., Shih, I. M., McMaster, M. T., Yeo, C. J., & Maitra, A. (2005). HLA-G upregulation in pre-malignant and malignant lesions of the gastrointestinal tract. International Journal of Gastrointestinal Cancer, 35(1), 15-23.

HLA-G upregulation in pre-malignant and malignant lesions of the gastrointestinal tract. / Hansel, Donna E.; Rahman, Ayman; Wilentz, Robb E.; Shih, Ie Ming; McMaster, Michael T.; Yeo, Charles J.; Maitra, Anriban.

In: International Journal of Gastrointestinal Cancer, Vol. 35, No. 1, 2005, p. 15-23.

Research output: Contribution to journalArticle

Hansel, DE, Rahman, A, Wilentz, RE, Shih, IM, McMaster, MT, Yeo, CJ & Maitra, A 2005, 'HLA-G upregulation in pre-malignant and malignant lesions of the gastrointestinal tract', International Journal of Gastrointestinal Cancer, vol. 35, no. 1, pp. 15-23.
Hansel, Donna E. ; Rahman, Ayman ; Wilentz, Robb E. ; Shih, Ie Ming ; McMaster, Michael T. ; Yeo, Charles J. ; Maitra, Anriban. / HLA-G upregulation in pre-malignant and malignant lesions of the gastrointestinal tract. In: International Journal of Gastrointestinal Cancer. 2005 ; Vol. 35, No. 1. pp. 15-23.
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