HLA-DR2a is the dominant restriction molecule for the cytotoxic T cell response to myelin basic protein in DR2Dw2 individuals

Dolores Jaraquemada, Roland Martin, Sandra Rosen-Bronson, Marjorie Flerlage, Henry F. McFarland, Eric O. Long

Research output: Contribution to journalArticle

Abstract

The HLA-DR2 restriction of the T cell response to myelin basic protein (MBP) was studied using murine L cells transfected with DRa and either DR2a or DR2b β-chain cDNA. DR2a and DR2b represent the two isotypic DRβ chains expressed in DR2Dw2 haplotypes. Eleven MBP-specific cytolytic T cell lines derived from patients with multiple sclerosis were isolated. Two of these cell lines recognized MBP-pulsed DR2-expressing L cell transfectants and four of them could only recognize the L cells if the adhesion molecule ICAM-1 was expressed in addition to HLA-DR2. Five of the six lines were restricted by HLA-DR2a; one line recognized Ag in conjunction with DR2b, but only if ICAM-1 was coexpressed. The remaining five lines did not lyse MBP-pulsed L cells. The ability of the DR2b molecules on transfected cells to stimulate T cells was confirmed with DR2b-allospecific T cell clones. Although five MBP-specific lines were restricted by DR2a, they recognized different parts of the MBP molecule, as demonstrated by the presentation of shorter peptides. Thus, our results suggest that DR2a is a dominant restriction molecule in MBP-specific responses by DR2+ MS patients. The results also indicate that the reported heterogeneity in MBP epitopes recognized by DR2-restricted T cells, may not be due to the use of different restriction elements but, rather, to the binding of different MBP peptides to DR2a molecules.

Original languageEnglish (US)
Pages (from-to)2880-2885
Number of pages6
JournalJournal of Immunology
Volume145
Issue number9
StatePublished - Nov 1 1990
Externally publishedYes

Fingerprint

Myelin Basic Protein
T-Lymphocytes
HLA-DR2 Antigen
Intercellular Adhesion Molecule-1
Cell Line
Peptides
Cell Adhesion Molecules
Haplotypes
Multiple Sclerosis
Epitopes
Complementary DNA
Clone Cells

ASJC Scopus subject areas

  • Immunology

Cite this

Jaraquemada, D., Martin, R., Rosen-Bronson, S., Flerlage, M., McFarland, H. F., & Long, E. O. (1990). HLA-DR2a is the dominant restriction molecule for the cytotoxic T cell response to myelin basic protein in DR2Dw2 individuals. Journal of Immunology, 145(9), 2880-2885.

HLA-DR2a is the dominant restriction molecule for the cytotoxic T cell response to myelin basic protein in DR2Dw2 individuals. / Jaraquemada, Dolores; Martin, Roland; Rosen-Bronson, Sandra; Flerlage, Marjorie; McFarland, Henry F.; Long, Eric O.

In: Journal of Immunology, Vol. 145, No. 9, 01.11.1990, p. 2880-2885.

Research output: Contribution to journalArticle

Jaraquemada, D, Martin, R, Rosen-Bronson, S, Flerlage, M, McFarland, HF & Long, EO 1990, 'HLA-DR2a is the dominant restriction molecule for the cytotoxic T cell response to myelin basic protein in DR2Dw2 individuals', Journal of Immunology, vol. 145, no. 9, pp. 2880-2885.
Jaraquemada D, Martin R, Rosen-Bronson S, Flerlage M, McFarland HF, Long EO. HLA-DR2a is the dominant restriction molecule for the cytotoxic T cell response to myelin basic protein in DR2Dw2 individuals. Journal of Immunology. 1990 Nov 1;145(9):2880-2885.
Jaraquemada, Dolores ; Martin, Roland ; Rosen-Bronson, Sandra ; Flerlage, Marjorie ; McFarland, Henry F. ; Long, Eric O. / HLA-DR2a is the dominant restriction molecule for the cytotoxic T cell response to myelin basic protein in DR2Dw2 individuals. In: Journal of Immunology. 1990 ; Vol. 145, No. 9. pp. 2880-2885.
@article{28521b3a73f149d7b5ac72ab279fda3b,
title = "HLA-DR2a is the dominant restriction molecule for the cytotoxic T cell response to myelin basic protein in DR2Dw2 individuals",
abstract = "The HLA-DR2 restriction of the T cell response to myelin basic protein (MBP) was studied using murine L cells transfected with DRa and either DR2a or DR2b β-chain cDNA. DR2a and DR2b represent the two isotypic DRβ chains expressed in DR2Dw2 haplotypes. Eleven MBP-specific cytolytic T cell lines derived from patients with multiple sclerosis were isolated. Two of these cell lines recognized MBP-pulsed DR2-expressing L cell transfectants and four of them could only recognize the L cells if the adhesion molecule ICAM-1 was expressed in addition to HLA-DR2. Five of the six lines were restricted by HLA-DR2a; one line recognized Ag in conjunction with DR2b, but only if ICAM-1 was coexpressed. The remaining five lines did not lyse MBP-pulsed L cells. The ability of the DR2b molecules on transfected cells to stimulate T cells was confirmed with DR2b-allospecific T cell clones. Although five MBP-specific lines were restricted by DR2a, they recognized different parts of the MBP molecule, as demonstrated by the presentation of shorter peptides. Thus, our results suggest that DR2a is a dominant restriction molecule in MBP-specific responses by DR2+ MS patients. The results also indicate that the reported heterogeneity in MBP epitopes recognized by DR2-restricted T cells, may not be due to the use of different restriction elements but, rather, to the binding of different MBP peptides to DR2a molecules.",
author = "Dolores Jaraquemada and Roland Martin and Sandra Rosen-Bronson and Marjorie Flerlage and McFarland, {Henry F.} and Long, {Eric O.}",
year = "1990",
month = "11",
day = "1",
language = "English (US)",
volume = "145",
pages = "2880--2885",
journal = "Journal of Immunology",
issn = "0022-1767",
publisher = "American Association of Immunologists",
number = "9",

}

TY - JOUR

T1 - HLA-DR2a is the dominant restriction molecule for the cytotoxic T cell response to myelin basic protein in DR2Dw2 individuals

AU - Jaraquemada, Dolores

AU - Martin, Roland

AU - Rosen-Bronson, Sandra

AU - Flerlage, Marjorie

AU - McFarland, Henry F.

AU - Long, Eric O.

PY - 1990/11/1

Y1 - 1990/11/1

N2 - The HLA-DR2 restriction of the T cell response to myelin basic protein (MBP) was studied using murine L cells transfected with DRa and either DR2a or DR2b β-chain cDNA. DR2a and DR2b represent the two isotypic DRβ chains expressed in DR2Dw2 haplotypes. Eleven MBP-specific cytolytic T cell lines derived from patients with multiple sclerosis were isolated. Two of these cell lines recognized MBP-pulsed DR2-expressing L cell transfectants and four of them could only recognize the L cells if the adhesion molecule ICAM-1 was expressed in addition to HLA-DR2. Five of the six lines were restricted by HLA-DR2a; one line recognized Ag in conjunction with DR2b, but only if ICAM-1 was coexpressed. The remaining five lines did not lyse MBP-pulsed L cells. The ability of the DR2b molecules on transfected cells to stimulate T cells was confirmed with DR2b-allospecific T cell clones. Although five MBP-specific lines were restricted by DR2a, they recognized different parts of the MBP molecule, as demonstrated by the presentation of shorter peptides. Thus, our results suggest that DR2a is a dominant restriction molecule in MBP-specific responses by DR2+ MS patients. The results also indicate that the reported heterogeneity in MBP epitopes recognized by DR2-restricted T cells, may not be due to the use of different restriction elements but, rather, to the binding of different MBP peptides to DR2a molecules.

AB - The HLA-DR2 restriction of the T cell response to myelin basic protein (MBP) was studied using murine L cells transfected with DRa and either DR2a or DR2b β-chain cDNA. DR2a and DR2b represent the two isotypic DRβ chains expressed in DR2Dw2 haplotypes. Eleven MBP-specific cytolytic T cell lines derived from patients with multiple sclerosis were isolated. Two of these cell lines recognized MBP-pulsed DR2-expressing L cell transfectants and four of them could only recognize the L cells if the adhesion molecule ICAM-1 was expressed in addition to HLA-DR2. Five of the six lines were restricted by HLA-DR2a; one line recognized Ag in conjunction with DR2b, but only if ICAM-1 was coexpressed. The remaining five lines did not lyse MBP-pulsed L cells. The ability of the DR2b molecules on transfected cells to stimulate T cells was confirmed with DR2b-allospecific T cell clones. Although five MBP-specific lines were restricted by DR2a, they recognized different parts of the MBP molecule, as demonstrated by the presentation of shorter peptides. Thus, our results suggest that DR2a is a dominant restriction molecule in MBP-specific responses by DR2+ MS patients. The results also indicate that the reported heterogeneity in MBP epitopes recognized by DR2-restricted T cells, may not be due to the use of different restriction elements but, rather, to the binding of different MBP peptides to DR2a molecules.

UR - http://www.scopus.com/inward/record.url?scp=0025044023&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0025044023&partnerID=8YFLogxK

M3 - Article

C2 - 1698864

AN - SCOPUS:0025044023

VL - 145

SP - 2880

EP - 2885

JO - Journal of Immunology

JF - Journal of Immunology

SN - 0022-1767

IS - 9

ER -