TY - JOUR
T1 - HLA antigens, alleles and haplotypes among the Yup'ik Alaska natives
T2 - Report of the ASHI Minority Workshops, part II
AU - Leffell, Mary S.
AU - Fallin, M. Daniele
AU - Erlich, Henry A.
AU - Fernandez-Vĩna, Marcelo
AU - Hildebrand, William H.
AU - Mack, Steven J.
AU - Zachary, Andrea A.
N1 - Funding Information:
Recognizing a need for thorough definition and characterization of the human leukocyte antigen (HLA) system among minority populations, the American Society for Histocompatibility and Immunogenetics (ASHI) initiated a series of workshops that were funded in part by the National Institute of Allergy and Infectious Diseases, the National Institutes of Health in 1992. The workshop goals were to identify antigens and alleles within each population and to determine allele and haplotype frequencies. Studies have been conducted among three broadly defined groups: African-Americans, Hispanic/Latino Americans, and Native Americans/Alaska Natives. The results for African-Americans have been previously reported [1] . We present here the results from a study among the Central Yup’ik Eskimo, Alaska Natives of the Yukon Kuskokwim (YK) Delta.
Funding Information:
The authors sincerely thank the following Yukon Kuskokwim Health Corporation personnel for their assistance and support of this project: Joseph A. Klejka, M.D., Medical Director; Allen M. Joseph, Director, Tribal & Program Support Services; Bonnie Himshoot and Jack Crow, CHP Training Coordinators; and Community Health Aides–Beth Alexie, Victor Bell, Anna David, Grace Friendly, Sue Hoeldt, Gerald Hunt, Anna John, Norma Shorty, and Anna Tinker. This work was sponsored in part by NIH contract NO1-A1-82514.
PY - 2002
Y1 - 2002
N2 - As part of the American Society for Histocompatibility and Immunogenetics coordinated studies among minority populations, human leukocyte antigen (HLA) alleles were defined for 460 volunteer Yup'ik Eskimos from the Yukon Kuskokwim delta region of southwestern Alaska. The study group included 252 adults with no other first-degree relatives and 48 informative nuclear families. Full Yupik ancestry through both maternal and paternal grandparents was claimed by 81.1% of participants. HLA-A, -B, -Cw, -DRB1, and -DQB1 alleles were determined by SBT, SSOP, reverse SSOP, and/or RSCA according to the protocols of five participating laboratories. Polymorphism was limited with 3-6 alleles comprising > 80% of the alleles observed at each locus. Homozygosity was high, particularly at the HLA-A and -DQB1 loci, with 36.6% and 44% of individuals having a single allele defined at these respective loci. HLA-A, -B, and -DRB1 alleles were in Hardy-Weinberg equilibrium, whereas HLA-Cw and -DQB1 alleles gave significant deviation (p = 0.002; 0.005). Significant linkage disequilibrium (p ≤ 0.00001) was observed in all pairwise evaluations. A new Cw*0806 allele was observed in high linkage disequilibrium with B*4801(Δ = 0.099; Δrel = 1.0). Three extended haplotypes were found to have frequencies > 5%, the most prevalent being A*2402; B*4801; DRBI*0401; DQB1*0301 (0.0933). Comparison of available class I data indicate that the Yup'ik share several common alleles with other Native American populations, including: A*2402, *0206, *6801; B*1501, *2705, *3501, *4002, *4801, *5101; and Cw*0202, *0304, *0401. Comparisons of class II data also confirm a close relationship of the Yup'ik to two other Eskimo populations, .Siberian and East Greenland Eskimos. DRBI*0401 and *1101, which occur in high frequency among these Eskimo populations, but not in other Native Americans, were also prevalent among the Yup'ik, with respective frequencies of 0.232 and 0.107.
AB - As part of the American Society for Histocompatibility and Immunogenetics coordinated studies among minority populations, human leukocyte antigen (HLA) alleles were defined for 460 volunteer Yup'ik Eskimos from the Yukon Kuskokwim delta region of southwestern Alaska. The study group included 252 adults with no other first-degree relatives and 48 informative nuclear families. Full Yupik ancestry through both maternal and paternal grandparents was claimed by 81.1% of participants. HLA-A, -B, -Cw, -DRB1, and -DQB1 alleles were determined by SBT, SSOP, reverse SSOP, and/or RSCA according to the protocols of five participating laboratories. Polymorphism was limited with 3-6 alleles comprising > 80% of the alleles observed at each locus. Homozygosity was high, particularly at the HLA-A and -DQB1 loci, with 36.6% and 44% of individuals having a single allele defined at these respective loci. HLA-A, -B, and -DRB1 alleles were in Hardy-Weinberg equilibrium, whereas HLA-Cw and -DQB1 alleles gave significant deviation (p = 0.002; 0.005). Significant linkage disequilibrium (p ≤ 0.00001) was observed in all pairwise evaluations. A new Cw*0806 allele was observed in high linkage disequilibrium with B*4801(Δ = 0.099; Δrel = 1.0). Three extended haplotypes were found to have frequencies > 5%, the most prevalent being A*2402; B*4801; DRBI*0401; DQB1*0301 (0.0933). Comparison of available class I data indicate that the Yup'ik share several common alleles with other Native American populations, including: A*2402, *0206, *6801; B*1501, *2705, *3501, *4002, *4801, *5101; and Cw*0202, *0304, *0401. Comparisons of class II data also confirm a close relationship of the Yup'ik to two other Eskimo populations, .Siberian and East Greenland Eskimos. DRBI*0401 and *1101, which occur in high frequency among these Eskimo populations, but not in other Native Americans, were also prevalent among the Yup'ik, with respective frequencies of 0.232 and 0.107.
KW - Alaska native
KW - HLA alleles
KW - HLA haplotypes
KW - Yup'ik Eskimo
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U2 - 10.1016/S0198-8859(02)00415-9
DO - 10.1016/S0198-8859(02)00415-9
M3 - Article
C2 - 12072196
AN - SCOPUS:0035985448
SN - 0198-8859
VL - 63
SP - 614
EP - 625
JO - Human Immunology
JF - Human Immunology
IS - 7
ER -