HLA-A01-, -A03-, and -A024-binding nanomeric epitopes in polyomavirus BK large T antigen

Bala Ramaswami, Iulia Popescu, Camila Macedo, Diana Metes, Marta Bueno, Adriana Zeevi, Ron Shapiro, Raphael P Viscidi, Parmjeet S. Randhawa

Research output: Contribution to journalArticle

Abstract

Polyomavirus BK (BKV) infections are increasingly recognized. The development of immune-monitoring strategies against BKV requires definition of antigenic epitopes. Bioinformatic algorithms were used to identify 60 BKV large T-antigen (LT-Ag) peptides predicted to bind HLA class I alleles. In vitro peptide binding was used to select a subset of 19 peptides for interferon (IFN)-γ ELISPOT assays in 13 healthy subjects and 12 kidney transplant recipients. Four A01-, nine A03-, and five A24-binding immunogenic peptides were identified in 1 to 3 (14-67%) tested subjects in each group. BKV epitope sequences were identical to homologous JC virus sequences for 3 of 19 peptides and homologous SV40 sequences for 5 of 19 peptides. Homology modeling localized these epitopes to the helicase, origin of DNA binding, or J domains, respectively. In conclusion, we have identified multiple 9-mer BKV LT-Ag-derived immunogenic epitopes that bind HLA-A01, -A03, or -A24 molecules. Sequence alignments indicate that two epitopes, FLICKGVNK and RYWLFKGPI, are common to BKV, JC virus, and SV40 virus.

Original languageEnglish (US)
Pages (from-to)722-728
Number of pages7
JournalHuman Immunology
Volume70
Issue number9
DOIs
StatePublished - Sep 2009

Fingerprint

Polyomavirus Transforming Antigens
Epitopes
Peptides
JC Virus
Viral Tumor Antigens
BK Virus
DNA Helicases
Enzyme-Linked Immunospot Assay
Immunologic Monitoring
Simian virus 40
Sequence Alignment
Sequence Homology
Computational Biology
Interferons
Healthy Volunteers
Alleles
Kidney
Infection

Keywords

  • BK virus
  • Epitopes
  • Immunity
  • Large T antigen

ASJC Scopus subject areas

  • Immunology
  • Immunology and Allergy

Cite this

Ramaswami, B., Popescu, I., Macedo, C., Metes, D., Bueno, M., Zeevi, A., ... Randhawa, P. S. (2009). HLA-A01-, -A03-, and -A024-binding nanomeric epitopes in polyomavirus BK large T antigen. Human Immunology, 70(9), 722-728. https://doi.org/10.1016/j.humimm.2009.05.003

HLA-A01-, -A03-, and -A024-binding nanomeric epitopes in polyomavirus BK large T antigen. / Ramaswami, Bala; Popescu, Iulia; Macedo, Camila; Metes, Diana; Bueno, Marta; Zeevi, Adriana; Shapiro, Ron; Viscidi, Raphael P; Randhawa, Parmjeet S.

In: Human Immunology, Vol. 70, No. 9, 09.2009, p. 722-728.

Research output: Contribution to journalArticle

Ramaswami, B, Popescu, I, Macedo, C, Metes, D, Bueno, M, Zeevi, A, Shapiro, R, Viscidi, RP & Randhawa, PS 2009, 'HLA-A01-, -A03-, and -A024-binding nanomeric epitopes in polyomavirus BK large T antigen', Human Immunology, vol. 70, no. 9, pp. 722-728. https://doi.org/10.1016/j.humimm.2009.05.003
Ramaswami, Bala ; Popescu, Iulia ; Macedo, Camila ; Metes, Diana ; Bueno, Marta ; Zeevi, Adriana ; Shapiro, Ron ; Viscidi, Raphael P ; Randhawa, Parmjeet S. / HLA-A01-, -A03-, and -A024-binding nanomeric epitopes in polyomavirus BK large T antigen. In: Human Immunology. 2009 ; Vol. 70, No. 9. pp. 722-728.
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