HIV-DNA priming alters T cell responses to HIV-adenovirus vaccine even when responses to DNA are undetectable

Stephen C. De Rosa, Evan P. Thomas, John Bui, Yunda Huang, Allan DeCamp, Cecilia Morgan, Spyros A. Kalams, Georgia D. Tomaras, Rama Akondy, Rafi Ahmed, Chuen Yen Lau, Barney S. Graham, Gary J. Nabel, M. Juliana McElrath

Research output: Contribution to journalArticlepeer-review

Abstract

Many candidate HIV vaccines are designed to primarily elicit T cell responses. Although repeated immunization with the same vaccine boosts Ab responses, the benefit for T cell responses is ill defined.We compared two immunization regimens that include the same recombinant adenoviral serotype 5 (rAd5) boost. Repeated homologous rAd5 immunization fails to increase T cell responses, but increases gp140 Ab responses 10-fold. DNA prime, as compared with rAd5 prime, directs long-term memory CD8+ T cells toward a terminally differentiated effector memory phenotype with cytotoxic potential. Based on the kinetics of activated cells measured directly ex vivo, the DNAvaccination primes for both CD4+ and CD8+ T cells, despite the lack of detection of the latter until after the boost. These results suggest that heterologous prime-boost combinations have distinct immunological advantages over homologous prime-boosts and suggest that the effect of DNA on subsequent boosting may not be easily detectable directly after the DNA vaccination.

Original languageEnglish (US)
Pages (from-to)3391-3401
Number of pages11
JournalJournal of Immunology
Volume187
Issue number6
DOIs
StatePublished - Sep 15 2011
Externally publishedYes

ASJC Scopus subject areas

  • Immunology

Fingerprint Dive into the research topics of 'HIV-DNA priming alters T cell responses to HIV-adenovirus vaccine even when responses to DNA are undetectable'. Together they form a unique fingerprint.

Cite this