HIV-1 Vpr suppresses the cytomegalovirus promoter in a CRL4(DCAF1) E3 ligase independent manner

Xianjun Liu, Haoran Guo, Hong Wang, Richard Bryan Markham, Wei Wei, Xiao Fang Yu

Research output: Contribution to journalArticle

Abstract

Although the Vpr protein of human immunodeficiency virus type 1 (HIV-1) has been shown to act as a transcriptional activator of the HIV-1 LTR and certain host genes, the current study demonstrates that it can also function as a potent inhibitor of the cytomegalovirus (CMV) promoter. Previous studies have shown that the cell cycle arrest and apoptotic functions of Vpr required recruitment of the CRL4(DCAF1) E3 ligase, but this complex is shown not to be required for inhibition of the CMV promoter. We identified conserved sites (A30/V31) from diverse Vpr from HIV/SIV that were critical for blocking the CMV promoter activity. Interestingly, the Vpr mutant A30S/V31S protein also impaired the ability of Vpr to down-regulate transcription of the host UNG2 gene. Our findings shed light on the dual functions of Vpr on the transcription of HIV-1, other viruses and host genes which may contribute to viral replication and disease progression in vivo.

Original languageEnglish (US)
Pages (from-to)214-219
Number of pages6
JournalBiochemical and Biophysical Research Communications
Volume459
Issue number2
DOIs
StatePublished - Apr 3 2015

Fingerprint

Ubiquitin-Protein Ligases
Cytomegalovirus
Viruses
HIV-1
Genes
Transcription
vpr Gene Products
HIV Long Terminal Repeat
Virus Diseases
Cell Cycle Checkpoints
Disease Progression
Down-Regulation
Cells
HIV
Proteins

Keywords

  • CMV
  • HIV-1
  • Transcriptional regulation
  • UNG2
  • Vpr

ASJC Scopus subject areas

  • Biochemistry
  • Biophysics
  • Cell Biology
  • Molecular Biology
  • Medicine(all)

Cite this

HIV-1 Vpr suppresses the cytomegalovirus promoter in a CRL4(DCAF1) E3 ligase independent manner. / Liu, Xianjun; Guo, Haoran; Wang, Hong; Markham, Richard Bryan; Wei, Wei; Yu, Xiao Fang.

In: Biochemical and Biophysical Research Communications, Vol. 459, No. 2, 03.04.2015, p. 214-219.

Research output: Contribution to journalArticle

Liu, Xianjun ; Guo, Haoran ; Wang, Hong ; Markham, Richard Bryan ; Wei, Wei ; Yu, Xiao Fang. / HIV-1 Vpr suppresses the cytomegalovirus promoter in a CRL4(DCAF1) E3 ligase independent manner. In: Biochemical and Biophysical Research Communications. 2015 ; Vol. 459, No. 2. pp. 214-219.
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