HIV-1 envelope glycoprotein trimers display open quaternary conformation when bound to the gp41 membrane-proximal external-region-directed broadly neutralizing antibody Z13e1

Audray K. Harris, Alberto Bartesaghi, Jacqueline L S Milne, Sriram Subramaniam

Research output: Contribution to journalArticlepeer-review

Abstract

We describe cryo-electron microscopic studies of the interaction between the ectodomain of the trimeric HIV-1 envelope glycoprotein (Env) and Z13e1, a broadly neutralizing antibody that targets the membrane-proximal external region (MPER) of the gp41 subunit. We show that Z13e1-bound Env displays an open quaternary conformation similar to the CD4-bound conformation. Our results support the idea that MPER-directed antibodies, such as Z13e1, block viral entry by interacting with Env at a step after CD4 activation.

Original languageEnglish (US)
Pages (from-to)7191-7196
Number of pages6
JournalJournal of Virology
Volume87
Issue number12
DOIs
StatePublished - Jun 2013
Externally publishedYes

ASJC Scopus subject areas

  • Immunology
  • Virology

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