HIV-1 DNA is detected in bone marrow populations containing CD4 + T cells but is not found in purified CD34 + hematopoietic progenitor cells in most patients on antiretroviral therapy

Christine Durand, Gabriel Ghiaur, Janet M Siliciano, S. Alireza Rabi, Evelyn E. Eisele, Maria Salgado, Liang Shan, Jun F. Lai, Hao Zhang, Joseph Bernard Margolick, Richard J Jones, Joel E. Gallant, Richard F Ambinder, Robert F Siliciano

Research output: Contribution to journalArticle

Abstract

Identifying cellular reservoirs of human immunodeficiency virus type 1 (HIV-1) in patients on antiretroviral therapy (ART) is critical to finding a cure for HIV-1. In addition to resting CD4 + T cells, CD34 + hematopoietic progenitor cells have been proposed as another reservoir. We obtained bone marrow aspirates from 11 patients on ART who had undetectable plasma HIV-1 RNA. HIV-1 DNA was detected in CD4 + T cells from peripheral blood in all patients and from bone marrow cellular fractions containing T cells in most patients. We did not find HIV-1 DNA in highly purified CD34 + populations using either a sensitive real-time polymerase chain reaction assay or a coculture assay for replication-competent HIV-1.

Original languageEnglish (US)
Pages (from-to)1014-1018
Number of pages5
JournalJournal of Infectious Diseases
Volume205
Issue number6
DOIs
Publication statusPublished - Mar 15 2012

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ASJC Scopus subject areas

  • Infectious Diseases
  • Immunology and Allergy

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