HIV-1 DNA is detected in bone marrow populations containing CD4 + T cells but is not found in purified CD34 + hematopoietic progenitor cells in most patients on antiretroviral therapy

Christine M. Durand, Gabriel Ghiaur, Janet D. Siliciano, S. Alireza Rabi, Evelyn E. Eisele, Maria Salgado, Liang Shan, Jun F. Lai, Hao Zhang, Joseph Margolick, Richard J. Jones, Joel E. Gallant, Richard F. Ambinder, Robert F. Siliciano

Research output: Contribution to journalArticlepeer-review

Abstract

Identifying cellular reservoirs of human immunodeficiency virus type 1 (HIV-1) in patients on antiretroviral therapy (ART) is critical to finding a cure for HIV-1. In addition to resting CD4 + T cells, CD34 + hematopoietic progenitor cells have been proposed as another reservoir. We obtained bone marrow aspirates from 11 patients on ART who had undetectable plasma HIV-1 RNA. HIV-1 DNA was detected in CD4 + T cells from peripheral blood in all patients and from bone marrow cellular fractions containing T cells in most patients. We did not find HIV-1 DNA in highly purified CD34 + populations using either a sensitive real-time polymerase chain reaction assay or a coculture assay for replication-competent HIV-1.

Original languageEnglish (US)
Pages (from-to)1014-1018
Number of pages5
JournalJournal of Infectious Diseases
Volume205
Issue number6
DOIs
StatePublished - Mar 15 2012

ASJC Scopus subject areas

  • Immunology and Allergy
  • Infectious Diseases

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