HIV-1 Diversity, Drug-Resistant Mutations, and Viral Evolution among High-Risk Individuals in Phase II HIV Vaccine Trial Sites in Southern China

Haiyan Qi, Ke Zhao, Fei Xu, Xuzhao Zhang, Zhiyong Zhang, Li Yang, Chunling Li, Xu Liang, Weigui Guo, Shihai Chen, Zhihao Liu, Wenyan Zhang, Xiao Fang Yu

Research output: Contribution to journalArticle

Abstract

HIV-1 prevalence in Guangxi, China, has been growing since 1996, when the first case was reported. Over half of HIV-1 positive patients in Guangxi Province were injecting drug users (IDUs), possibly because of the province's location near drug-trafficking routes. Since a phase II HIV vaccine trial is ongoing there, a current characterization of the subtypes of HIV-1 among IDUs in Guangxi would provide critical information for future HIV vaccine trials, as well as further control and prevention of HIV-1 transmission. Thus, we conducted a molecular epidemiological investigation of HIV-1 samples from 2008-2010 among IDUs in multiple cities in Guangxi Province. Our results, based on the gag/pol fragment, indicated a very high proportion (78.47%) of HIV-1 CRF08_BC recombinants, some CRF01_AE (15.38%) recombinants, and a low proportion of CRF07_BC (6.15%) recombinants among the IDUs. The high proportion of CRF08 HIV-1 strains among recent IDUs matches the vaccine candidate constructs. However, future vaccine development should also incorporate CRF01-targeted vaccine candidates. Distinct Env sequence evolution patterns were observed for CRF08_BC and CRF01_AE, indicating that different local selection pressures have been exerted on these two HIV-1 subtypes. Unique drug-resistant mutations were also detected, and our data indicate that HIV treatment programs should consider pre-existing drug-resistant mutations.

Original languageEnglish (US)
Article numbere68656
JournalPloS one
Volume8
Issue number7
DOIs
StatePublished - Jul 15 2013

ASJC Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology(all)
  • Agricultural and Biological Sciences(all)
  • General

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    Qi, H., Zhao, K., Xu, F., Zhang, X., Zhang, Z., Yang, L., Li, C., Liang, X., Guo, W., Chen, S., Liu, Z., Zhang, W., & Yu, X. F. (2013). HIV-1 Diversity, Drug-Resistant Mutations, and Viral Evolution among High-Risk Individuals in Phase II HIV Vaccine Trial Sites in Southern China. PloS one, 8(7), [e68656]. https://doi.org/10.1371/journal.pone.0068656