HIV-1 coat protein gp120-induced increases in levels of intrasynaptosomal calcium

Avindra Nath, Rodolfo A. Padua, Jonathan D. Geiger

Research output: Contribution to journalArticle

Abstract

The effects of human immunodeficiency virus type-1 (HIV-1) coat protein gp120 on levels of intrasynaptosomal calcium ([Ca2+]i) were determined in rat cortical synaptosomes. gp120 at concentrations of ≥ 400 pM, significantly (P <0.05) increased levels of [Ca2+]i. Treatment with 20 mM KCl, reduced the concentrations of gp120 necessary to produce significant (P <0.001) increases in [Ca2+]i. gp120-evoked increases in [Ca2+]i were prevented either by treatment with dantrolene or by removal of extracellular calcium with BATPA. The peak levels of gp120-induced increases in [Ca2+]i were not affected by calcium channel blockers lanthanum and nicardipine, by glutamate receptor antagonists MK-801 and NBQX, or by removal of endogenous glutamate with glutamate dehydrogenase. gp120-induced [Ca2+]i increases in presynaptic terminals may play a role in HIV-mediated effects in the central nervous system.

Original languageEnglish (US)
Pages (from-to)200-206
Number of pages7
JournalBrain Research
Volume678
Issue number1-2
DOIs
StatePublished - Apr 24 1995
Externally publishedYes

Fingerprint

Capsid Proteins
HIV-1
Dantrolene
Calcium
Nicardipine
Lanthanum
Excitatory Amino Acid Antagonists
Glutamate Dehydrogenase
Dizocilpine Maleate
Synaptosomes
Presynaptic Terminals
Calcium Channel Blockers
Glutamic Acid
Central Nervous System
HIV
2,3-dioxo-6-nitro-7-sulfamoylbenzo(f)quinoxaline

Keywords

  • Calcium
  • Calcium channel blocker
  • Dantrolene
  • Glutamate
  • gp120
  • HIV-1
  • Synaptosome

ASJC Scopus subject areas

  • Developmental Biology
  • Molecular Biology
  • Clinical Neurology
  • Neuroscience(all)

Cite this

HIV-1 coat protein gp120-induced increases in levels of intrasynaptosomal calcium. / Nath, Avindra; Padua, Rodolfo A.; Geiger, Jonathan D.

In: Brain Research, Vol. 678, No. 1-2, 24.04.1995, p. 200-206.

Research output: Contribution to journalArticle

Nath, Avindra ; Padua, Rodolfo A. ; Geiger, Jonathan D. / HIV-1 coat protein gp120-induced increases in levels of intrasynaptosomal calcium. In: Brain Research. 1995 ; Vol. 678, No. 1-2. pp. 200-206.
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