Histopathologic and immunohistochemical studies of keratoglobus

Beeran Meghpara, Hiroshi Nakamura, Geeta K. Vemuganti, Somasheila I. Murthy, Joel Sugar, Beatrice Y J T Yue, Deepak P. Edward

Research output: Contribution to journalArticlepeer-review


Objective: To examine histopathologic and immunohistochemical features of human corneal buttons from patients who developed keratoglobus. Methods: Nine corneal buttons were obtained during penetrating keratoplasty from patients with keratoglobus. Histologic features were examined using hematoxylin-eosin-stained sections. Immunohistochemical staining for α1-proteinase inhibitor, Sp1, and matrix metalloproteinases 1, 2, and 3 was performed, with 2 normal and 2 corneal sections with keratoconus as controls. Results: Hematoxylin-eosin staining revealed diffuse stromal thinning and focal disruptions in Bowman's layer in all keratoglobus specimens. Similar abnormal immunostaining results for α1-proteinase inhibitor and Sp1 were detected in keratoglobus and keratoconus at their respective active disease sites. Immunostaining for matrix metalloproteinases 1, 2, and 3 was significantly more intense in corneas with keratoglobus than in normal controls. Matrix metalloproteinase staining intensity was especially prominent in areas where the underlying Bowman's layer was disrupted. Conclusions: Histological features in our keratoglobus specimens are consistent with previous reports. The similarities in immunohistochemical labeling between keratoglobus and keratoconus suggest that these entities may share common mechanisms that are involved in stromal thinning.

Original languageEnglish (US)
Pages (from-to)1029-1035
Number of pages7
JournalArchives of Ophthalmology
Issue number8
StatePublished - Aug 2009
Externally publishedYes

ASJC Scopus subject areas

  • Ophthalmology


Dive into the research topics of 'Histopathologic and immunohistochemical studies of keratoglobus'. Together they form a unique fingerprint.

Cite this