Histone H3 exerts a key function in mitotic checkpoint control

Jianjun Luo, Xinjing Xu, Hana Hall, Edel M. Hyland, Jef D. Boeke, Tony Hazbun, Min Hao Kuo

Research output: Contribution to journalArticle

Abstract

It has been firmly established that many interphase nuclear functions, including transcriptional regulation, are regulated by chromatin and histones. How mitotic progression and quality control might be influenced by histones is less well characterized. We show that histone H3 plays a crucial role in activating the spindle assembly checkpoint in response to a defect in mitosis. Prior to anaphase, all chromosomes must attach to spindles emanating from the opposite spindle pole bodies. The tension between sister chromatids generated by the poleward pulling force is an integral part of chromosome biorientation. Lack of tension due to erroneous attachment activates the spindle assembly checkpoint, which corrects the mistakes and ensures segregation fidelity. A histone H3 mutation impairs the ability of yeast cells to activate the checkpoint in a tensionless crisis, leading to missegregation and aneuploidy. The defects in tension sensing result directly from an attenuated H3-Sgo1p interaction essential for pericentric recruitment of Sgo1p. Reinstating the pericentric enrichment of Sgo1p alleviates the mitotic defects. Histone H3, and hence the chromatin, is thus a key factor transmitting the tension status to the spindle assembly checkpoint.

Original languageEnglish (US)
Pages (from-to)537-549
Number of pages13
JournalMolecular and Cellular Biology
Volume30
Issue number2
DOIs
StatePublished - Jan 2010

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M Phase Cell Cycle Checkpoints
Histones
Chromatin
Chromosomes
Spindle Pole Bodies
Anaphase
Chromatids
Interphase
Aneuploidy
Mitosis
Quality Control
Yeasts
Mutation

ASJC Scopus subject areas

  • Molecular Biology
  • Cell Biology

Cite this

Luo, J., Xu, X., Hall, H., Hyland, E. M., Boeke, J. D., Hazbun, T., & Kuo, M. H. (2010). Histone H3 exerts a key function in mitotic checkpoint control. Molecular and Cellular Biology, 30(2), 537-549. https://doi.org/10.1128/MCB.00980-09

Histone H3 exerts a key function in mitotic checkpoint control. / Luo, Jianjun; Xu, Xinjing; Hall, Hana; Hyland, Edel M.; Boeke, Jef D.; Hazbun, Tony; Kuo, Min Hao.

In: Molecular and Cellular Biology, Vol. 30, No. 2, 01.2010, p. 537-549.

Research output: Contribution to journalArticle

Luo, J, Xu, X, Hall, H, Hyland, EM, Boeke, JD, Hazbun, T & Kuo, MH 2010, 'Histone H3 exerts a key function in mitotic checkpoint control', Molecular and Cellular Biology, vol. 30, no. 2, pp. 537-549. https://doi.org/10.1128/MCB.00980-09
Luo J, Xu X, Hall H, Hyland EM, Boeke JD, Hazbun T et al. Histone H3 exerts a key function in mitotic checkpoint control. Molecular and Cellular Biology. 2010 Jan;30(2):537-549. https://doi.org/10.1128/MCB.00980-09
Luo, Jianjun ; Xu, Xinjing ; Hall, Hana ; Hyland, Edel M. ; Boeke, Jef D. ; Hazbun, Tony ; Kuo, Min Hao. / Histone H3 exerts a key function in mitotic checkpoint control. In: Molecular and Cellular Biology. 2010 ; Vol. 30, No. 2. pp. 537-549.
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