Histologic localization of sodium fluorescein in human ocular tissues

Robert T. McMahon, Mark O M Tso, Ian W. McLean

Research output: Contribution to journalArticle

Abstract

We studied the distribution pattern of sodium fluorescein in human eyes microscopically. The ciliary body showed early and diffuse leakage, with staining of the basement membrane of the nonpigmented ciliary epithelium, indicating movement of fluorescein from the ciliary body into the aqueous humor. After five minutes the iris stroma stained with fluorescein, probably from the aqueous humor. The retinal vessels and retinal pigment epithelium were impermeable to fluorescein. Corresponding to the background fluorescence seen in fluorescein angiography, fluorescence was present in Bruch's membrane and in the stroma of the inner one third of the choroid. Drusen stained most intensely in areas of greatest PAS positivity. Early fluorescence of the optic disk was the result of intravascular perfusion of the dye. Minimal diffusion of fluorescein from the fenestrated choroidal vessels across the border tissue of Elschnig into the peripheral optic nerve bundles was observed. Late fluorescence of the optic disk was due mainly to fluorescein staining of the lamina scleralis and glial columns.

Original languageEnglish (US)
Pages (from-to)1058-1065
Number of pages8
JournalAmerican Journal of Ophthalmology
Volume80
Issue number6
StatePublished - 1975

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Fluorescein
Fluorescence
Ciliary Body
Aqueous Humor
Optic Disk
Bruch Membrane
Staining and Labeling
Retinal Vessels
Choroid
Retinal Pigment Epithelium
Fluorescein Angiography
Iris
Optic Nerve
Peripheral Nerves
Basement Membrane
Neuroglia
Coloring Agents
Epithelium
Perfusion

ASJC Scopus subject areas

  • Ophthalmology

Cite this

Histologic localization of sodium fluorescein in human ocular tissues. / McMahon, Robert T.; Tso, Mark O M; McLean, Ian W.

In: American Journal of Ophthalmology, Vol. 80, No. 6, 1975, p. 1058-1065.

Research output: Contribution to journalArticle

McMahon, Robert T. ; Tso, Mark O M ; McLean, Ian W. / Histologic localization of sodium fluorescein in human ocular tissues. In: American Journal of Ophthalmology. 1975 ; Vol. 80, No. 6. pp. 1058-1065.
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