Histochemical localization of superoxide production in experimental autoimmune uveitis

David C. Gritz; Carolina Montes; Lily R. Atalla; Guey Shuang Wu; Alex Sevanian; Narsing A. Rao

doi:10.3109/02713689109020328

Histochemical localization of superoxide production in experimental autoimmune uveitis

David C. Gritz, Carolina Montes, Lily R. Atalla, Guey Shuang Wu, Alex Sevanian, Narsing A. Rao

Research output: Contribution to journal › Article › peer-review

32 Scopus citations

Abstract

Although the presence and role of oxygen reactive species in uveal inflammation is the subject of intense investigation, there is little direct evidence that oxygen metabolites are present at the site of inflammation. We used the nitroblue tetrazolium test for superoxide to determine production of this oxygen reactive species in experimental autoimmune uveitis (EAU). The choroidal tissues of animals with this disease contained intracellular, blue-staining granules. Most of the positive staining cells appeared to be polymorphonuclear leukocytes. This localization of superoxide in EAU is further evidence of the generation of oxygen reactive species in uveal inflammation.

Original language	English (US)
Pages (from-to)	927-931
Number of pages	5
Journal	Current Eye Research
Volume	10
Issue number	10
DOIs	https://doi.org/10.3109/02713689109020328
State	Published - 1991
Externally published	Yes

ASJC Scopus subject areas

Ophthalmology
Sensory Systems
Cellular and Molecular Neuroscience

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title = "Histochemical localization of superoxide production in experimental autoimmune uveitis",

abstract = "Although the presence and role of oxygen reactive species in uveal inflammation is the subject of intense investigation, there is little direct evidence that oxygen metabolites are present at the site of inflammation. We used the nitroblue tetrazolium test for superoxide to determine production of this oxygen reactive species in experimental autoimmune uveitis (EAU). The choroidal tissues of animals with this disease contained intracellular, blue-staining granules. Most of the positive staining cells appeared to be polymorphonuclear leukocytes. This localization of superoxide in EAU is further evidence of the generation of oxygen reactive species in uveal inflammation.",

author = "Gritz, {David C.} and Carolina Montes and Atalla, {Lily R.} and Wu, {Guey Shuang} and Alex Sevanian and Rao, {Narsing A.}",

note = "Funding Information: ACKNO WLEDGEM EN'I'S Supported in part by grant EY 05662 from the National Institutes of Health, and a grant from Research to Prevent Blindness, Inc. Presented in part in the thesis (N.A.R.) required for membership in the American Ophthalmological Society. Ann Dawson, medical editor, reviewed this manuscript.",

year = "1991",

doi = "10.3109/02713689109020328",

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AU - Gritz, David C.

AU - Montes, Carolina

AU - Atalla, Lily R.

AU - Wu, Guey Shuang

AU - Sevanian, Alex

AU - Rao, Narsing A.

N1 - Funding Information: ACKNO WLEDGEM EN'I'S Supported in part by grant EY 05662 from the National Institutes of Health, and a grant from Research to Prevent Blindness, Inc. Presented in part in the thesis (N.A.R.) required for membership in the American Ophthalmological Society. Ann Dawson, medical editor, reviewed this manuscript.

PY - 1991

Y1 - 1991

N2 - Although the presence and role of oxygen reactive species in uveal inflammation is the subject of intense investigation, there is little direct evidence that oxygen metabolites are present at the site of inflammation. We used the nitroblue tetrazolium test for superoxide to determine production of this oxygen reactive species in experimental autoimmune uveitis (EAU). The choroidal tissues of animals with this disease contained intracellular, blue-staining granules. Most of the positive staining cells appeared to be polymorphonuclear leukocytes. This localization of superoxide in EAU is further evidence of the generation of oxygen reactive species in uveal inflammation.

AB - Although the presence and role of oxygen reactive species in uveal inflammation is the subject of intense investigation, there is little direct evidence that oxygen metabolites are present at the site of inflammation. We used the nitroblue tetrazolium test for superoxide to determine production of this oxygen reactive species in experimental autoimmune uveitis (EAU). The choroidal tissues of animals with this disease contained intracellular, blue-staining granules. Most of the positive staining cells appeared to be polymorphonuclear leukocytes. This localization of superoxide in EAU is further evidence of the generation of oxygen reactive species in uveal inflammation.

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Histochemical localization of superoxide production in experimental autoimmune uveitis

Abstract

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